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Title

Increased uptake and improved intracellular survival of a teicoplanin-resistant mutant of methicillin-resistant Staphylococcus aureus in non-professional phagocytes

Authors
Huggler, Elzbieta
Published in Chemotherapy. 2009, vol. 55, no. 3, p. 183-188
Abstract BACKGROUND/AIMS: Endogenous development of glycopeptide-intermediate resistance is linked to multiple genetic and phenotypic changes in clinical and laboratory isolates of Staphylococcus aureus. This study evaluated endocytic uptake and intracellular survival of a teicoplanin-resistant derivative of S. aureus in a human epithelial cell line, and compared these to the isogenic teicoplanin-susceptible parent or a spontaneously derived, susceptible revertant. METHODS: Endocytic uptake of teicoplanin-resistant and teicoplanin-susceptible strains by human embryonic kidney 293 cells was estimated by a lysostaphin protection assay. Differential intracellular survival of all S. aureus strains from 2 to 24 h was evaluated by colony-forming unit counts of Triton X-100-lysed 293 cells, following lysostaphin inactivation. RESULTS: Endocytic uptake of the teicoplanin-resistant strain increased by approximately 4-fold over its teicoplanin-susceptible counterparts. Furthermore, the teicoplanin-resistant strain showed an 11-fold increase in intracellular colony-forming unit counts from 2 to 24 h, compared to its teicoplanin-susceptible counterparts that showed marginal (<2-fold) changes during the same time period. Infected host cells showed no significant viability loss at 24 h, as assessed by Trypan blue dye exclusion. CONCLUSIONS: Intracellular location might confer a significant fitness benefit to glycopeptide-intermediate isolates of methicillin-resistant S. aureus and further protect them from cell wall-active antibiotics whose intracellular activity is limited.
Keywords Anti-Bacterial Agents/ pharmacologyCell LineDrug Resistance, BacterialHumansLysostaphin/metabolismMethicillin-Resistant Staphylococcus aureus/ drug effects/genetics/isolation &PurificationMicrobial Sensitivity TestsMutationPhagocytes/ microbiologyTeicoplanin/ pharmacologyTime Factors
Identifiers
PMID: 19407446
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Research group Acquisition et expression de facteurs de virulence chez Staphylococcus aureus (86)
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(ISO format)
RENZONI, Adriana Maria et al. Increased uptake and improved intracellular survival of a teicoplanin-resistant mutant of methicillin-resistant Staphylococcus aureus in non-professional phagocytes. In: Chemotherapy, 2009, vol. 55, n° 3, p. 183-188. https://archive-ouverte.unige.ch/unige:7545

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Deposited on : 2010-06-21

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