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Scientific article
OA Policy
English

NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets

Contributors

Zhou, Bin; Li, Yan; Belser, Jessica A; Pearce, Melissa B; Schmolke, Mirco; Subba, Anju X; Shi, Zhengli; Zaki, Sherif R; Blau, Dianna M; García-Sastre, Adolfo; Tumpey, Terrence M; Wentworth, David E

Published in

Vaccine, vol. 28, no. 50, p. 8015-8025

Publication date

2010

Abstract

Although vaccines against influenza A virus are the most effective method to combat infection, it is clear that their production needs to be accelerated and their efficacy improved. We generated live attenuated human influenza A vaccines (LAIVs) by rationally engineering mutations directly into the genome of a pandemic-H1N1 virus. Two LAIVs (NS1-73 and NS1-126) were based on the success of LAIVs for animal influenza A viruses. A third candidate (NSΔ5) is a unique NS-mutant that has never been used as a LAIV. The vaccine potential of each LAIV was determined through analysis of attenuation, interferon production, immunogenicity, and their ability to protect mice and ferrets. This study demonstrates that NSΔ5 is an ideal LAIV candidate, provides important information on the effects that different NS mutations have on the pandemic-H1N1 virus and shows that LAIVs can be engineered directly from the genomes of emerging/circulating influenza A viruses.

Keywords

  • Animals
  • Dogs
  • Female
  • Ferrets
  • Genetic Engineering
  • HEK293 Cells
  • Humans
  • Influenza A Virus, H1N1 Subtype/immunology/physiology
  • Influenza Vaccines/biosynthesis/genetics/immunology
  • Interferon-beta/immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections/prevention & control
  • Pandemics/prevention & control
  • Sequence Deletion
  • Vaccines, Attenuated/biosynthesis/genetics/immunology
  • Viral Nonstructural Proteins/genetics/immunology
  • Virus Replication

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

ZHOU, Bin et al. NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets. In: Vaccine, 2010, vol. 28, n° 50, p. 8015–8025. doi: 10.1016/j.vaccine.2010.08.106

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0264-410X
650views
1618downloads

Technical informations

Creation

22/09/2015 16:01:00

First validation

22/09/2015 16:01:00

Update

14/03/2023 23:37:58

Status update

14/03/2023 23:37:58

Last indexation

31/10/2024 01:18:55
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