Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60

Pittet, Didier; Di Virgilio, F.; Pozzan, T.; Monod, Antoinette; Lew, Daniel Pablo

doi:10.1016/S0021-9258(18)77294-3

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Title		Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60
Authors		Pittet, Didier Di Virgilio, F. Pozzan, T. Monod, Antoinette Lew, Daniel Pablo
Published in		The Journal of biological chemistry. 1990, vol. 265, no. 24, p. 14256-14263
Abstract		The effects of plasma membrane depolarization on cytosolic free calcium ([Ca2+]i) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) generation were investigated in the human promyelocytic cell line HL-60 differentiated with either dimethyl sulfoxide or retinoic acid into neutrophil-like cells. Increases in [Ca2+]i and accumulation of Ins(1,4,5)P3 were triggered by two chemoattractants fMet-Leu-Phe and leukotriene B4. Plasma membrane potential was depolarized by isoosmotic substitution of NaCl with KCl, by the pore-forming ionophore gramicidin D, or by long term treatment with ouabain. Both Ca2+ mobilization from intracellular stores and Ca2+ influx across the plasma membrane were reduced by prior depolarization of plasma membrane potential regardless of the procedure employed to collapse it. Agonist-induced generation of Ins(1,4,5)P3 was also reduced in parallel in pre-depolarized HL-60 cells. The present findings provide further evidence suggesting that plasma membrane potential can be an important modulator of agonist-activated second messenger generation in myelocytic cells.
Keywords		Calcium/metabolism — Cell Line — Cell Membrane/drug effects/ physiology — Cytosol/metabolism — Dimethyl Sulfoxide/pharmacology — Egtazic Acid/pharmacology — Gramicidin/pharmacology — Humans — Inositol 1,4,5-Trisphosphate/metabolism — Ionomycin/pharmacology — Kinetics — Leukemia, Promyelocytic, Acute — Membrane Potentials/drug effects — N-Formylmethionine Leucyl-Phenylalanine/pharmacology — Ouabain/pharmacology — Potassium Chloride/pharmacology — Second Messenger Systems/drug effects — Tretinoin/pharmacology — Tumor Cells, Cultured/drug effects/ physiology
Identifiers		DOI: 10.1016/S0021-9258(18)77294-3 PMID: 1696945
Full text		Article - Limited access to UNIGE Other version: http://www.jbc.org/content/265/24/14256.full.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research group		Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
Citation (ISO format)		PITTET, Didier et al. Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60. In: Journal of Biological Chemistry, 1990, vol. 265, n° 24, p. 14256-14263. doi: 10.1016/S0021-9258(18)77294-3 https://archive-ouverte.unige.ch/unige:7494