en
Scientific article
English

Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60

Published inThe Journal of biological chemistry, vol. 265, no. 24, p. 14256-14263
Publication date1990
Abstract

The effects of plasma membrane depolarization on cytosolic free calcium ([Ca2+]i) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) generation were investigated in the human promyelocytic cell line HL-60 differentiated with either dimethyl sulfoxide or retinoic acid into neutrophil-like cells. Increases in [Ca2+]i and accumulation of Ins(1,4,5)P3 were triggered by two chemoattractants fMet-Leu-Phe and leukotriene B4. Plasma membrane potential was depolarized by isoosmotic substitution of NaCl with KCl, by the pore-forming ionophore gramicidin D, or by long term treatment with ouabain. Both Ca2+ mobilization from intracellular stores and Ca2+ influx across the plasma membrane were reduced by prior depolarization of plasma membrane potential regardless of the procedure employed to collapse it. Agonist-induced generation of Ins(1,4,5)P3 was also reduced in parallel in pre-depolarized HL-60 cells. The present findings provide further evidence suggesting that plasma membrane potential can be an important modulator of agonist-activated second messenger generation in myelocytic cells.

Keywords
  • Calcium/metabolism
  • Cell Line
  • Cell Membrane/drug effects/ physiology
  • Cytosol/metabolism
  • Dimethyl Sulfoxide/pharmacology
  • Egtazic Acid/pharmacology
  • Gramicidin/pharmacology
  • Humans
  • Inositol 1,4,5-Trisphosphate/metabolism
  • Ionomycin/pharmacology
  • Kinetics
  • Leukemia, Promyelocytic, Acute
  • Membrane Potentials/drug effects
  • N-Formylmethionine Leucyl-Phenylalanine/pharmacology
  • Ouabain/pharmacology
  • Potassium Chloride/pharmacology
  • Second Messenger Systems/drug effects
  • Tretinoin/pharmacology
  • Tumor Cells, Cultured/drug effects/ physiology
Citation (ISO format)
PITTET, Didier et al. Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60. In: The Journal of biological chemistry, 1990, vol. 265, n° 24, p. 14256–14263. doi: 10.1016/S0021-9258(18)77294-3
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ISSN of the journal0021-9258
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