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Title

Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60

Authors
Di Virgilio, F.
Pozzan, T.
Published in Journal of Biological Chemistry. 1990, vol. 265, no. 24, p. 14256-14263
Abstract The effects of plasma membrane depolarization on cytosolic free calcium ([Ca2+]i) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) generation were investigated in the human promyelocytic cell line HL-60 differentiated with either dimethyl sulfoxide or retinoic acid into neutrophil-like cells. Increases in [Ca2+]i and accumulation of Ins(1,4,5)P3 were triggered by two chemoattractants fMet-Leu-Phe and leukotriene B4. Plasma membrane potential was depolarized by isoosmotic substitution of NaCl with KCl, by the pore-forming ionophore gramicidin D, or by long term treatment with ouabain. Both Ca2+ mobilization from intracellular stores and Ca2+ influx across the plasma membrane were reduced by prior depolarization of plasma membrane potential regardless of the procedure employed to collapse it. Agonist-induced generation of Ins(1,4,5)P3 was also reduced in parallel in pre-depolarized HL-60 cells. The present findings provide further evidence suggesting that plasma membrane potential can be an important modulator of agonist-activated second messenger generation in myelocytic cells.
Keywords Calcium/metabolismCell LineCell Membrane/drug effects/ physiologyCytosol/metabolismDimethyl Sulfoxide/pharmacologyEgtazic Acid/pharmacologyGramicidin/pharmacologyHumansInositol 1,4,5-Trisphosphate/metabolismIonomycin/pharmacologyKineticsLeukemia, Promyelocytic, AcuteMembrane Potentials/drug effectsN-Formylmethionine Leucyl-Phenylalanine/pharmacologyOuabain/pharmacologyPotassium Chloride/pharmacologySecond Messenger Systems/drug effectsTretinoin/pharmacologyTumor Cells, Cultured/drug effects/ physiology
Identifiers
PMID: 1696945
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jbc.org/content/265/24/14256.full.pdf
Structures
Research group Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
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PITTET, Didier et al. Correlation between plasma membrane potential and second messenger generation in the promyelocytic cell line HL-60. In: Journal of Biological Chemistry, 1990, vol. 265, n° 24, p. 14256-14263. https://archive-ouverte.unige.ch/unige:7494

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Deposited on : 2010-06-21

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