UNIGE document Scientific Article
previous document  unige:7471  next document
add to browser collection

DksA represses ribosomal gene transcription in Pseudomonas aeruginosa by interacting with RNA polymerase on ribosomal promoters

Comte, Rachel
Published in Molecular Microbiology. 2005, vol. 56, no. 4, p. 1087-1102
Abstract In Escherichia coli transcription of ribosomal RNA (rRNA) is regulated by the H-NS and Fis proteins, as well as by the small signal molecule ppGpp and the initiating nucleotides. During amino acid starvation, the concentration of ppGpp increases, and binding of this alarmone to RNA polymerase (RNAP) leads to inhibition of rRNA transcription, a regulatory event called stringent response. Here we show that in Pseudomonas aeruginosa DksA, a protein with pleiotropic effects, is a negative regulator of rRNA transcription both during exponential growth and stringent conditions. A dksA mutant overexpresses rRNA, without being affected in the production of ppGpp. Cell-fractionation and chromosome immunoprecipitation experiments demonstrate that DksA is associated with DNA, in particular with promoters of ribosomal genes in vivo. The binding to rRNA promoters specifically increases during stringent response, and correlates with the binding of RNAP to these regions. Moreover DksA can be copurified with RNAP subunits in vivo. DNA band shift experiments show that DksA, in synergy with ppGpp, increases the binding of RNAP to ribosomal promoters. Therefore DksA might be a new regulator of rRNA transcription in P. aeruginosa.
Keywords Bacterial Proteins/genetics/isolation & purification/ metabolismDNA, BacterialDNA-Directed RNA Polymerases/chemistry/ metabolismEscherichia coli Proteins/genetics/metabolismGene Expression Regulation, BacterialPromoter Regions, GeneticProtein Subunits/genetics/isolation & purification/metabolismPseudomonas aeruginosa/genetics/metabolismRNA, Ribosomal/genetics/ metabolismTranscription, Genetic
PMID: 15853892
Full text
(ISO format)
PERRON, Karl, COMTE, Rachel, VAN DELDEN, Christian. DksA represses ribosomal gene transcription in Pseudomonas aeruginosa by interacting with RNA polymerase on ribosomal promoters. In: Molecular Microbiology, 2005, vol. 56, n° 4, p. 1087-1102. https://archive-ouverte.unige.ch/unige:7471

222 hits

0 download


Deposited on : 2010-06-21

Export document
Format :
Citation style :