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Gene therapy targeting cord blood-derived CD34+ cells from HIV-exposed infants: preclinical studies

Li, X
Kang, D
Law, P
Spector, S A
Ho, A D
Wong-Staal, F
Published in Gene Therapy. 1998, vol. 5, no. 2, p. 233-9
Abstract Hematopoietic CD34+ cells from placental and umbilical cord blood (PUCB) can be valuable vehicles for gene therapy of immunodeficiencies and genetic disorders. We have conducted preclinical studies towards the treatment of HIV-1-infected infants with genetically 'immunized' CD34+ cells derived from PUCB using anti-HIV-1 hairpin ribozyme genes. PUCB was collected from 10 newborns of HIV-1-positive mothers. CD34+ cells were enriched with a modified procedure using Dynal immunomagnetic beads and chymopapain, stimulated with stem cell factor (SCF), IL-3 and IL-6, and transduced using cell-free recombinant retroviral vector (MJT) expressing a ribozyme against the U5 region of HIV-1. No significant differences were observed in the growth pattern of CD34+ cells from normal infants, HIV-1 exposed infants or infants confirmed to be infected by HIV-1. The transduction efficiency of the CD34+ cells from all the infants was also comparable. MJT-transduced CD34+ cells from an HIV-1-infected infant were maintained in a liquid culture system for 4 weeks, and the progeny macrophage cells were challenged with the monocyte-tropic laboratory strain, HIV-Bal, or the HIV-1 isolate from the infant's mother. Significant inhibition of virus replication was observed in ribozyme-transduced cells. Thus, we have demonstrated efficient and stable gene transfer into progenitor cells from the cord blood of HIV-1-exposed or -infected infants and shown that protection from HIV-1 infection was conferred to the progeny cells produced by CD34+ cells transduced with the anti-HIV ribozyme gene construct.
Keywords Antigens, CD34Cells, CulturedFetal Blood/immunologyGene ExpressionGene TargetingGenetic Therapy/methodsHIV Infections/immunology/prevention & controlHIV-1/geneticsHumansInfant, NewbornRNA, CatalyticStem Cells/immunology
PMID: 9578843
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Article (Published version) (90 Kb) - public document Free access
Research group Etiologie des pneumonies et marqueurs inflammatoires chez l'enfant fébrile (183)
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LI, X et al. Gene therapy targeting cord blood-derived CD34+ cells from HIV-exposed infants: preclinical studies. In: Gene Therapy, 1998, vol. 5, n° 2, p. 233-9. https://archive-ouverte.unige.ch/unige:74576

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