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Scientific article
English

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Published inJournal of clinical microbiology, vol. 41, no. 2, p. 826-830
Publication date2003
Abstract

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

Keywords
  • Animals
  • Arthrodermataceae/ isolation & purification
  • Base Sequence
  • Cats
  • DNA, Fungal/analysis
  • Dogs
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 28S/ analysis/genetics
  • Reagent Kits, Diagnostic
  • Sequence Homology, Nucleic Acid
Citation (ISO format)
BESCHER NINET, Béatrice Alice et al. Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit. In: Journal of clinical microbiology, 2003, vol. 41, n° 2, p. 826–830. doi: 10.1128/jcm.41.2.826-830.2003
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ISSN of the journal0095-1137
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