Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Bescher Ninet, Béatrice Alice; Jan, Isabelle; Bontems, Olympia; Lechenne, Barbara; Jousson, Olivier; Panizzon, Renato; Lew, Daniel Pablo; Monod, Michel

doi:10.1128/jcm.41.2.826-830.2003

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Title		Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit
Authors		Bescher Ninet, Béatrice Alice Jan, Isabelle Bontems, Olympia Lechenne, Barbara Jousson, Olivier Panizzon, Renato Lew, Daniel Pablo Monod, Michel
Published in		Journal of clinical microbiology. 2003, vol. 41, no. 2, p. 826-830
Abstract		We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.
Keywords		Animals — Arthrodermataceae/ isolation & purification — Base Sequence — Cats — DNA, Fungal/analysis — Dogs — Humans — Molecular Sequence Data — Polymerase Chain Reaction — RNA, Ribosomal, 28S/ analysis/genetics — Reagent Kits, Diagnostic — Sequence Homology, Nucleic Acid
Identifiers		DOI: 10.1128/jcm.41.2.826-830.2003 PMID: 12574293
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC149666/pdf/0644.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		BESCHER NINET, Béatrice Alice et al. Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit. In: Journal of clinical microbiology, 2003, vol. 41, n° 2, p. 826-830. doi: 10.1128/jcm.41.2.826-830.2003 https://archive-ouverte.unige.ch/unige:7444