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Scientific article
English

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

ContributorsBescher Ninet, Béatrice Alice; Jan, Isabelle; Bontems, Olympia; Lechenne, Barbara; Jousson, Olivier; Panizzon, Renato; Lew, Daniel Pablo; Monod, Michel
Published inJournal of clinical microbiology, vol. 41, no. 2, p. 826-830
Publication date2003
Abstract

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

Keywords
  • Animals
  • Arthrodermataceae/ isolation & purification
  • Base Sequence
  • Cats
  • DNA, Fungal/analysis
  • Dogs
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 28S/ analysis/genetics
  • Reagent Kits, Diagnostic
  • Sequence Homology, Nucleic Acid
Affiliation entities
Citation (ISO format)
BESCHER NINET, Béatrice Alice et al. Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit. In: Journal of clinical microbiology, 2003, vol. 41, n° 2, p. 826–830. doi: 10.1128/jcm.41.2.826-830.2003
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Additional URL for this publicationhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC149666/pdf/0644.pdf
Journal ISSN0095-1137
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