UNIGE document Scientific Article
previous document  unige:74286  next document
add to browser collection

Uncoupling histone turnover from transcription-associated histone H3 modifications

Published in Nucleic Acids Research. 2015, vol. 43, no. 8, p. 3972-85
Abstract Transcription in eukaryotes is associated with two major changes in chromatin organization. Firstly, nucleosomal histones are continuously replaced by new histones, an event that in yeast occurs predominantly at transcriptionally active promoters. Secondly, histones become modified post-translationally at specific lysine residues. Some modifications, including histone H3 trimethylation at lysine 4 (H3K4me3) and acetylation at lysines 9 (H3K9ac) and 14 (H3K14ac), are specifically enriched at active promoters where histones exchange, suggesting a possible causal relationship. Other modifications accumulate within transcribed regions and one of them, H3K36me3, is thought to prevent histone exchange. Here we explored the relationship between these four H3 modifications and histone turnover at a few selected genes. Using lysine-to-arginine mutants and a histone exchange assay, we found that none of these modifications plays a major role in either promoting or preventing histone turnover. Unexpectedly, mutation of H3K56, whose acetylation occurs prior to chromatin incorporation, had an effect only when introduced into the nucleosomal histone. Furthermore, we used various genetic approaches to show that histone turnover can be experimentally altered with no major consequence on the H3 modifications tested. Together, these results suggest that transcription-associated histone turnover and H3 modification are two correlating but largely independent events.
PMID: 25845593
Full text
Article (Published version) (746 Kb) - public document Free access
Research group Régulation de l'expression des gènes du virus de l'hépatite B (294)
Project FNS: 31003A_127384
(ISO format)
FERRARI, Paolo, STRUBIN, Michel. Uncoupling histone turnover from transcription-associated histone H3 modifications. In: Nucleic Acids Research, 2015, vol. 43, n° 8, p. 3972-85. doi: 10.1093/nar/gkv282 https://archive-ouverte.unige.ch/unige:74286

500 hits



Deposited on : 2015-07-22

Export document
Format :
Citation style :