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Title

The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus

Authors
Majcherczyk, P. A.
McKenna, Therese
Published in FEMS Microbiology Letters. 2006, vol. 255, no. 2, p. 233-239
Abstract To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More important changes in MALDI-TOF MS spectra were found with strains differing in methicillin than in teicoplanin resistance. In comparison, very minor or no changes were recorded in pulsed-field gel electrophoresis profiles or peptidoglycan muropeptide digest patterns of these strains, respectively. MALDI-TOF MS might be useful to detect subtle strain-specific differences in ionizable components released from bacterial surfaces and not from their peptidoglycan network.
Keywords Anti-Bacterial Agents/ pharmacologyBacterial Typing Techniques/methodsDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldHumansMethicillin ResistanceMuramidase/metabolismPeptides/analysisPeptidoglycan/chemistry/metabolismSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/ methodsStaphylococcus aureus/chemistry/ classification/drug effects/geneticsTeicoplanin/ pharmacology
Identifiers
PMID: 16448500
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MAJCHERCZYK, P. A. et al. The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus. In: FEMS Microbiology Letters, 2006, vol. 255, n° 2, p. 233-239. https://archive-ouverte.unige.ch/unige:7422

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Deposited on : 2010-06-21

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