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Lipopolysaccharide as shield and receptor for R-pyocin-mediated killing in Pseudomonas aeruginosa

Published in Journal of bacteriology. 2010, vol. 192, no. 7, p. 1921-1928
Abstract Pseudomonas aeruginosa produces three different types of bacteriocins: the soluble S-pyocins and the bacteriophage-like F- and R-pyocins. R-pyocins kill susceptible bacteria of the same or closely related species with high efficiency. Five different types of R-pyocins (R1- to R5-pyocins) have been described based on their killing spectra and tail fiber protein sequences. We analyzed the distribution of R-pyocin genes in a collection of clinical P. aeruginosa isolates. We found similar percentages of isolates not containing R-pyocins (28%) and isolates containing genes encoding R1-pyocins (25%), R2-pyocins (17%), and R5-pyocins (29%). The R-pyocin-deficient isolates were susceptible to R1-, R2-, and R5-pyocins, while most R2- and R5- pyocin producers were resistant. Determination of the O serotypes revealed that the R-pyocin-susceptible isolates belonged to serotypes O1, O3, and O6, while the R-pyocin-resistant isolates were serotype O10, O11, and O12 isolates. We hypothesized that O-serotype-specific lipopolysaccharide (LPS) packaging densities may account for the distinct accessibilities of R-pyocins to their receptors at the cell surface. Using genetically defined LPS mutants, we showed that the l-Rha residue and two distinct d-Glc residues of the outer core are part of the receptor sites for R1-, R2-, and R5-pyocins, respectively. To illustrate R-pyocin-mediated intraspecies biological warfare, we monitored the population dynamics of two different R-pyocin-producing P. aeruginosa clones of sequential respiratory isolates obtained from a colonized patient. The results of this study highlight the potential role of R-pyocins in shaping bacterial populations during host colonization and support use of these molecules as specific and potent bactericidal agents.
Keywords Anti-Bacterial Agents/metabolism/ toxicityAntibiosisHumansLipopolysaccharides/chemistry/genetics/ metabolismModels, MolecularMutationO Antigens/analysisPseudomonas Infections/microbiologyPseudomonas aeruginosa/genetics/isolation & purification/ physiologyPyocins/metabolism/ toxicitySerotyping
PMID: 20118263
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Other version: http://jb.asm.org/cgi/reprint/192/7/1921.pdf
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KOHLER, Thilo, DONNER, Viviane Pascale, VAN DELDEN, Christian. Lipopolysaccharide as shield and receptor for R-pyocin-mediated killing in Pseudomonas aeruginosa. In: Journal of bacteriology, 2010, vol. 192, n° 7, p. 1921-1928. doi: 10.1128/JB.01459-09 https://archive-ouverte.unige.ch/unige:7386

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Deposited on : 2010-06-21

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