UNIGE document Scientific Article
previous document  unige:7380  next document
add to browser collection

Positive control of the two-component RcsC/B signal transduction network by DjlA: a member of the DnaJ family of molecular chaperones in Escherichia coli

Georgopoulos, C.
Published in Molecular microbiology. 1997, vol. 25, no. 5, p. 913-931
Abstract The membrane-anchored DjIA protein represents the third member of the DnaJ 'J-domain' family of Escherichia coli that includes DnaJ and CbpA. DjIA possesses a J-domain at its extreme C-terminus but shares no additional homology with DnaJ. Our genetic analysis suggests that DjIA acts in concert with the RcsB/C two-component signal transduction system to augment induction of the cps (capsular polysaccharide) operon and synthesis of colanic acid mucoid capsule. The DjIA J-domain is essential for the observed stimulation of this pathway as deletion, or introduction of the mutation H233Q, within the highly conserved HPD tripeptide abolished all inducing activity. Deletion of the transmembrane anchor sequence also abolished all inducing activity. djIA is not an essential gene under all conditions tested, nor is it essential for mucoid capsule biosynthesis; however, strong overexpression leads to rapid loss of cell viability suggesting that the gene is normally tightly regulated. Northern analysis revealed that djIA message was extremely unstable but could be induced or stabilized in response to cold shock. The activation of the cps operon by DjIA is dependent upon both DnaK(Hsp70) and GrpE, and therefore we propose a role for DjIA, together with this chaperone machine, as a novel regulator of a two-component histidine kinase signal transduction pathway.
Keywords Amino Acid SequenceBacterial Capsules/geneticsBacterial Proteins/genetics/ physiologyCell Survival/genetics/physiologyChromosome MappingCloning, MolecularCold TemperatureEscherichia coli/genetics/physiologyEscherichia coli ProteinsGene Expression Regulation, BacterialGenetic Complementation TestGenetic Vectors/geneticsHSP40 Heat-Shock ProteinsHSP70 Heat-Shock Proteins/genetics/physiologyHeat-Shock Proteins/genetics/physiologyMolecular Chaperones/genetics/physiologyMolecular Sequence DataMultienzyme ComplexesMutation/genetics/physiologyPhosphoprotein PhosphatasesPromoter Regions, Genetic/genetics/physiologyProtein KinasesRNA, Messenger/analysis/geneticsSequence Homology, Amino AcidSignal Transduction/genetics/ physiologyTemperatureTranscription Factors/genetics/ physiologyTranscription, Genetic/genetics/physiology
PMID: 9364917
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
KELLEY, William, GEORGOPOULOS, C. Positive control of the two-component RcsC/B signal transduction network by DjlA: a member of the DnaJ family of molecular chaperones in Escherichia coli. In: Molecular microbiology, 1997, vol. 25, n° 5, p. 913-931. doi: 10.1111/j.1365-2958.1997.mmi527.x https://archive-ouverte.unige.ch/unige:7380

416 hits

0 download


Deposited on : 2010-06-21

Export document
Format :
Citation style :