The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone

Kelley, William; Georgopoulos, C.

doi:10.1073/pnas.94.8.3679

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Title		The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone
Authors		Kelley, William Georgopoulos, C.
Published in		Proceedings of the National Academy of Sciences of the United States of America. 1997, vol. 94, no. 8, p. 3679-3684
Abstract		The N-terminal 70 residue "J-domain" of the Escherichia coli DnaJ molecular chaperone is the defining and highly conserved feature of a large protein family. Based upon limited, yet significant, amino acid sequence homology to the J-domain, the DNA encoding the T/t common exon of the simian virus 40 (SV40), JC, or BK polyoma virus T antigen oncoproteins was used to construct J-domain replacement chimeras of the E. coli DnaJ chaperone. The virally encoded J-domains successfully substituted for the bacterial counterpart in vivo as shown by (i) complementation for viability at low and high temperature of a hypersensitive bacterial reporter strain, and (ii) the restoration of bacteriophage lambda plaque forming ability in the same strain. The amino acid change, H42Q, in the SV40 T/t and the JC virus T/t exon, which is positionally equivalent to the canonical dnaJ259 H33Q mutation within the E. coli J-domain, entirely abolished complementing activity. These results strongly suggest that the heretofore functionally undefined viral T/t common exon represents a bona fide J-domain that preserves critical features of the characteristic domain fold essential for J-domain interaction with the ATPase domain of the Hsp70 family. This finding has implications for the regulation of DNA tumor virus T antigens by molecular chaperones.
Keywords		Amino Acid Sequence — Antigens, Viral, Tumor/ genetics — BK Virus/ genetics/immunology — Escherichia coli/ genetics — Escherichia coli Proteins — Exons/ genetics — HSP40 Heat-Shock Proteins — Heat-Shock Proteins/ genetics — JC Virus/ genetics/immunology — Molecular Sequence Data — Sequence Alignment — Sequence Analysis — Simian virus 40/ genetics/immunology
Identifiers		DOI: 10.1073/pnas.94.8.3679 PMID: 9108037
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC20500/pdf/pq003679.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		KELLEY, William, GEORGOPOULOS, C. The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. In: Proceedings of the National Academy of Sciences of the United States of America, 1997, vol. 94, n° 8, p. 3679-3684. doi: 10.1073/pnas.94.8.3679 https://archive-ouverte.unige.ch/unige:7379