en
Scientific article
English

The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone

Publication date1997
Abstract

The N-terminal 70 residue "J-domain" of the Escherichia coli DnaJ molecular chaperone is the defining and highly conserved feature of a large protein family. Based upon limited, yet significant, amino acid sequence homology to the J-domain, the DNA encoding the T/t common exon of the simian virus 40 (SV40), JC, or BK polyoma virus T antigen oncoproteins was used to construct J-domain replacement chimeras of the E. coli DnaJ chaperone. The virally encoded J-domains successfully substituted for the bacterial counterpart in vivo as shown by (i) complementation for viability at low and high temperature of a hypersensitive bacterial reporter strain, and (ii) the restoration of bacteriophage lambda plaque forming ability in the same strain. The amino acid change, H42Q, in the SV40 T/t and the JC virus T/t exon, which is positionally equivalent to the canonical dnaJ259 H33Q mutation within the E. coli J-domain, entirely abolished complementing activity. These results strongly suggest that the heretofore functionally undefined viral T/t common exon represents a bona fide J-domain that preserves critical features of the characteristic domain fold essential for J-domain interaction with the ATPase domain of the Hsp70 family. This finding has implications for the regulation of DNA tumor virus T antigens by molecular chaperones.

Keywords
  • Amino Acid Sequence
  • Antigens, Viral, Tumor/ genetics
  • BK Virus/ genetics/immunology
  • Escherichia coli/ genetics
  • Escherichia coli Proteins
  • Exons/ genetics
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins/ genetics
  • JC Virus/ genetics/immunology
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis
  • Simian virus 40/ genetics/immunology
Citation (ISO format)
KELLEY, William, GEORGOPOULOS, C. The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. In: Proceedings of the National Academy of Sciences of the United States of America, 1997, vol. 94, n° 8, p. 3679–3684. doi: 10.1073/pnas.94.8.3679
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ISSN of the journal0027-8424
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