The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone

Kelley, William; Georgopoulos, C.

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Title		The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone
Authors		Kelley, William Georgopoulos, C.
Published in		Proceedings of the National Academy of Sciences. 1997, vol. 94, no. 8, p. 3679-3684
Abstract		The N-terminal 70 residue "J-domain" of the Escherichia coli DnaJ molecular chaperone is the defining and highly conserved feature of a large protein family. Based upon limited, yet significant, amino acid sequence homology to the J-domain, the DNA encoding the T/t common exon of the simian virus 40 (SV40), JC, or BK polyoma virus T antigen oncoproteins was used to construct J-domain replacement chimeras of the E. coli DnaJ chaperone. The virally encoded J-domains successfully substituted for the bacterial counterpart in vivo as shown by (i) complementation for viability at low and high temperature of a hypersensitive bacterial reporter strain, and (ii) the restoration of bacteriophage lambda plaque forming ability in the same strain. The amino acid change, H42Q, in the SV40 T/t and the JC virus T/t exon, which is positionally equivalent to the canonical dnaJ259 H33Q mutation within the E. coli J-domain, entirely abolished complementing activity. These results strongly suggest that the heretofore functionally undefined viral T/t common exon represents a bona fide J-domain that preserves critical features of the characteristic domain fold essential for J-domain interaction with the ATPase domain of the Hsp70 family. This finding has implications for the regulation of DNA tumor virus T antigens by molecular chaperones.
Keywords		Amino Acid Sequence — Antigens, Viral, Tumor/ genetics — BK Virus/ genetics/immunology — Escherichia coli/ genetics — Escherichia coli Proteins — Exons/ genetics — HSP40 Heat-Shock Proteins — Heat-Shock Proteins/ genetics — JC Virus/ genetics/immunology — Molecular Sequence Data — Sequence Alignment — Sequence Analysis — Simian virus 40/ genetics/immunology
Identifiers		DOI: 10.1073/pnas.94.8.3679 PMID: 9108037
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC20500/pdf/pq003679.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Citation (ISO format)		KELLEY, William, GEORGOPOULOS, C. The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. In: Proceedings of the National Academy of Sciences, 1997, vol. 94, n° 8, p. 3679-3684. https://archive-ouverte.unige.ch/unige:7379