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Title

The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone

Authors
Georgopoulos, C.
Published in Proceedings of the National Academy of Sciences. 1997, vol. 94, no. 8, p. 3679-3684
Abstract The N-terminal 70 residue "J-domain" of the Escherichia coli DnaJ molecular chaperone is the defining and highly conserved feature of a large protein family. Based upon limited, yet significant, amino acid sequence homology to the J-domain, the DNA encoding the T/t common exon of the simian virus 40 (SV40), JC, or BK polyoma virus T antigen oncoproteins was used to construct J-domain replacement chimeras of the E. coli DnaJ chaperone. The virally encoded J-domains successfully substituted for the bacterial counterpart in vivo as shown by (i) complementation for viability at low and high temperature of a hypersensitive bacterial reporter strain, and (ii) the restoration of bacteriophage lambda plaque forming ability in the same strain. The amino acid change, H42Q, in the SV40 T/t and the JC virus T/t exon, which is positionally equivalent to the canonical dnaJ259 H33Q mutation within the E. coli J-domain, entirely abolished complementing activity. These results strongly suggest that the heretofore functionally undefined viral T/t common exon represents a bona fide J-domain that preserves critical features of the characteristic domain fold essential for J-domain interaction with the ATPase domain of the Hsp70 family. This finding has implications for the regulation of DNA tumor virus T antigens by molecular chaperones.
Keywords Amino Acid SequenceAntigens, Viral, Tumor/ geneticsBK Virus/ genetics/immunologyEscherichia coli/ geneticsEscherichia coli ProteinsExons/ geneticsHSP40 Heat-Shock ProteinsHeat-Shock Proteins/ geneticsJC Virus/ genetics/immunologyMolecular Sequence DataSequence AlignmentSequence AnalysisSimian virus 40/ genetics/immunology
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PMID: 9108037
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KELLEY, William, GEORGOPOULOS, C. The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. In: Proceedings of the National Academy of Sciences, 1997, vol. 94, n° 8, p. 3679-3684. https://archive-ouverte.unige.ch/unige:7379

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Deposited on : 2010-06-21

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