UNIGE document Scientific Article
previous document  unige:7366  next document
add to browser collection
Title

Improved monitoring of cytomegalovirus infection after allogeneic hematopoietic stem cell transplantation by an ultrasensitive plasma DNA PCR assay

Authors
Kolarova, Lenka
Huguet, Saadia
show hidden authors show all authors [1 - 9]
Published in Journal of Clinical Microbiology. 2002, vol. 40, no. 11, p. 4251-4255
Abstract Cytomegalovirus (CMV) DNA amplification assays in plasma have shown limited sensitivity compared to the detection of pp65 antigen in leukocytes. Our goal was to increase the sensitivity of a commercial CMV DNA PCR quantitative assay. After modification, the new assay was able to reproducibly detect 20 CMV DNA copies/ml of plasma. We compared this new ultrasensitive PCR assay with the standard PCR and the pp65 test for CMV detection and quantification in 22 consecutive allogeneic hematopoietic stem cell recipients. CMV infection or reactivation was detected in 84 of 319 (26%) samples by the ultrasensitive PCR assay compared to 38 of 319 (12%) samples by the pp65 assay (P < 0.01). All samples positive by the pp65 assay were positive by the ultrasensitive PCR, and CMV episodes were detected on average 4 days earlier and 7 days later than the first and the last pp65-positive test, respectively. In addition, during CMV episodes, the ultrasensitive assay identified positive samples that were inconsistently detected by the pp65 assay. The ultrasensitive assay was also much more sensitive than the standard PCR, with 26 versus 12% of CMV DNA-positive samples (P < 0.01). This assay improved the monitoring of CMV infection or reactivation in hematopoietic allogeneic stem cell recipients.
Keywords AdolescentAdultChildCytomegalovirus/ isolation & purification/physiologyCytomegalovirus Infections/ virologyDNA, Viral/ bloodFemaleHematopoietic Stem Cell Transplantation/ adverse effectsHumansMaleMiddle AgedPhosphoproteins/bloodPolymerase Chain Reaction/ methodsReagent Kits, DiagnosticSensitivity and SpecificityTransplantation, Homologous/ adverse effectsViral Matrix Proteins/blood
Identifiers
PMID: 12409406
Full text
Structures
Citation
(ISO format)
KAISER, Laurent et al. Improved monitoring of cytomegalovirus infection after allogeneic hematopoietic stem cell transplantation by an ultrasensitive plasma DNA PCR assay. In: Journal of Clinical Microbiology, 2002, vol. 40, n° 11, p. 4251-4255. https://archive-ouverte.unige.ch/unige:7366

177 hits

0 download

Update

Deposited on : 2010-06-21

Export document
Format :
Citation style :