Scientific article

Resistance and virulence of Pseudomonas aeruginosa clinical strains overproducing the MexCD-OprJ efflux pump

Published inAntimicrobial agents and chemotherapy, vol. 52, no. 7, p. 2455-2462
Publication date2008

Since their initial description 2 decades ago, MexCD-OprJ-overproducing efflux mutants of Pseudomonas aeruginosa (also called nfxB mutants) have rarely been described in the clinical setting. Screening of 110 nonreplicate clinical isolates showing moderate resistance to ciprofloxacin (MIC from 0.5 microg/ml to 4 microg/ml) yielded only four mutants (3.6%) of that type harboring various alterations in the repressor gene nfxB. MexCD-OprJ upregulation correlated with an increased resistance to ciprofloxacin, cefepime, and chloramphenicol in most of the clinical strains, concomitant with a higher susceptibility to ticarcillin, aztreonam, imipenem, and aminoglycosides. Evidence was obtained that this increased susceptibility to aminoglycosides results from the impaired activity of efflux pump MexXY-OprM. Furthermore, MexCD-OprJ upregulation was found to impair bacterial growth and to have a strain-specific, variable impact on rhamnolipid, elastase, phospholipase C, and pyocyanin production. Review of patient files indicated that the four nfxB mutants were responsible for confirmed cases of infection and emerged during long-term therapy with ciprofloxacin. Taken together, these data show that, while rather infrequent among P. aeruginosa strains with low-level resistance to ciprofloxacin, MexCD-OprJ-overproducing mutants may be isolated after single therapy with fluoroquinolones and may be pathogenic.

  • Adult
  • Aged
  • Anti-Bacterial Agents/pharmacology
  • Bacterial Proteins/biosynthesis/genetics
  • Base Sequence
  • Ciprofloxacin/pharmacology
  • DNA Primers/genetics
  • DNA, Bacterial/genetics
  • DNA-Binding Proteins/biosynthesis/genetics
  • Drug Resistance, Bacterial/genetics/physiology
  • Female
  • Genes, Bacterial
  • Humans
  • Male
  • Membrane Proteins/biosynthesis/genetics
  • Membrane Transport Proteins/biosynthesis/genetics
  • Middle Aged
  • Molecular Sequence Data
  • Mutation
  • Pseudomonas Infections/drug therapy/microbiology
  • Pseudomonas aeruginosa/ drug effects/genetics/metabolism/ pathogenicity
  • Transcription Factors/biosynthesis/genetics
  • Virulence/genetics/physiology
Citation (ISO format)
JEANNOT, Katy et al. Resistance and virulence of Pseudomonas aeruginosa clinical strains overproducing the MexCD-OprJ efflux pump. In: Antimicrobial agents and chemotherapy, 2008, vol. 52, n° 7, p. 2455–2462. doi: 10.1128/AAC.01107-07
Updates (1)
ISSN of the journal0066-4804

Technical informations

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