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Role of intracellular calcium and protein kinase C in the endocytosis of transferrin and insulin by HL60 cells

Iacopetta, B.
Pozzan, T.
Gorden, P.
Published in The journal of cell biology. 1986, vol. 103, no. 3, p. 851-856
Abstract The role of the cytosolic free calcium concentration ([Ca2+]i) and of protein kinase C on the internalization of transferrin and insulin in the human promyelocytic cell line HL60 was investigated. [Ca2+]i was selectively monitored and manipulated by the use of the fluorescent Ca2+ indicator and buffer quin2, while receptor-ligand internalization was studied directly by quantitative electron microscope autoradiography. Decreasing the [Ca2+]i up to 10-fold below resting level had no effect on the internalization of transferrin or insulin. Similarly, a 10-fold elevation of the [Ca2+]i using the calcium ionophore ionomycin caused little or no change in the endocytosis of the two ligands. In contrast, activation of protein kinase C by phorbol myristate acetate markedly stimulated the internalization of both occupied and unoccupied transferrin receptors, even in cells with very low [Ca2+]i. The insulin receptor was found to behave differently in response to phorbol myristate acetate, however, in that only the occupied receptors were stimulated to internalize. We conclude that the [Ca2+]i plays only a minor role in regulating receptor-mediated endocytosis, whereas protein kinase C can selectively modulate receptor internalization depending on receptor type and occupancy.
Keywords Calcium/ physiologyCell LineEndocytosisHumansInsulin/ metabolismLeukemia, Myeloid, AcuteProtein Kinase C/ physiologyReceptor, Insulin/metabolismReceptors, Cell Surface/metabolismReceptors, TransferrinTetradecanoylphorbol Acetate/pharmacologyTransferrin/ metabolism
PMID: 3017998
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IACOPETTA, B. et al. Role of intracellular calcium and protein kinase C in the endocytosis of transferrin and insulin by HL60 cells. In: The Journal of Cell Biology, 1986, vol. 103, n° 3, p. 851-856. https://archive-ouverte.unige.ch/unige:7347

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Deposited on : 2010-06-21

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