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Title

Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide-specific antibody response and protective efficacy

Authors
Jakobsen, Håvard
Hannesdottir, Solveig
Bjarnarson, Stefania P
Schulz, Dominique
Trannoy, Emanuelle
Jonsdottir, Ingileif
Published in European Journal of Immunology. 2006, vol. 36, no. 2, p. 287-95
Abstract Immunization with a tetanus-protein (TT) pneumococcal polysaccharide (PPS) conjugate vaccine (Pnc1-TT) induces protective immunity against lethal pneumococcal infections in neonatal and infant mice, but anti-PPS IgG response and protective efficacy is lower than in adult mice. Here, we show that reduced antibody (Ab) response and protection against infections is directly related to impaired T cell response to the carrier. Whereas spleen cells from adult mice immunized with Pnc1-TT responded with proliferation and IFN-gamma secretion to in vitro stimulation with TT, spleen cells from neonatal and infant mice did not. However, significant, but age dependent, Th2-cytokine responses were observed in mice immunized with Pnc1-TT. Impaired IFN-gamma production upon TT-stimulation in vitro was also reflected in reduced IFN-gamma/IL-5 ratio. The IL--5 response correlated with IgG anti-PPS titers, and the lack of PPS Ab in the majority of neonatal mice was clearly associated with absence of carrier-specific IL-5 production. These results show that immunization with Pnc1-TT induces carrier-specific T cell responses that increase with age and determine the levels of PPS-specific Ab elicited. Whereas a weak and Th2-biased response was observed in neonatal mice, infant mice showed a mixed Th1-Th2 response as observed in adults.
Keywords Adjuvants, Immunologic/administration & dosageAging/immunologyAnimalsAnimals, NewbornAntibodies, Bacterial/immunologyAntibody Formation/immunologyImmunologic Memory/immunologyLymphocyte Activation/immunologyMicePneumococcal Vaccines/administration & dosage/immunologyPolysaccharides, Bacterial/administration & dosage/immunologyTetanus Toxoid/administration & dosage/immunologyTh1 Cells/immunologyTh2 Cells/immunologyVaccines, Conjugate/administration & dosage/immunology
Identifiers
PMID: 16385627
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Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
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JAKOBSEN, Håvard et al. Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide-specific antibody response and protective efficacy. In: European Journal of Immunology, 2006, vol. 36, n° 2, p. 287-95. https://archive-ouverte.unige.ch/unige:73129

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Deposited on : 2015-06-09

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