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Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model

Coudray, Alice
Prigent-Combaret, Claire
Bergeret, Evelyne
Perrin, Jackie
Reimmann, Cornelia
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Published in Cellular microbiology. 2008, vol. 10, no. 3, p. 729-40
Abstract The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa, and identified mutants with attenuated virulence towards Dictyostelium. These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa.
Keywords ATP-Binding Cassette Transporters/genetics/physiologyAnimalsBacterial Proteins/genetics/physiologyDNA Transposable ElementsDictyostelium/microbiologyDrosophilaFemaleHumansMaleMiceMutagenesis InsertionalPseudomonas Infections/microbiologyPseudomonas aeruginosa/genetics/pathogenicitySurvival AnalysisVirulence/geneticsVirulence Factors/genetics
PMID: 18042255
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Research groups Phagocytose (140)
Quorom-sensing et gènes de virulence (301)
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ALIBAUD, Laethitia et al. Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model. In: Cellular microbiology, 2008, vol. 10, n° 3, p. 729-40. doi: 10.1111/j.1462-5822.2007.01080.x https://archive-ouverte.unige.ch/unige:731

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Deposited on : 2009-02-09

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