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Title

Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons
Authors
Maco, Bohumil
Holtmaat, Anthony
Cantoni, Marco
Kreshuk, Anna
Straehle, Christoph N.
Hamprecht, Fred A.
Knott, Graham W.
Published in PLOS ONE. 2013, vol. 8, no. 2, e57405
Abstract Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently imaged in three dimensions with focused ion beam scanning electron microcopy. These neurites are then identified and reconstructed automatically from the image series using the latest segmentation algorithms. The fast and reliable imaging and reconstruction technique avoids any specific labeling to identify the features of interest in the electron microscope, and optimises their preservation and staining for 3D analysis.
Identifiers
PMID: 23468982
Full text
Article (Published version) (578 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales
Research group Les circuits neuronaux dans le néocortex des mammifères (855)
Projects
FNS: CRSI33-127289
FNS: 3100AO-120685
Citation
(ISO format) 		MACO, Bohumil et al. Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons. In: PLOS ONE, 2013, vol. 8, n° 2, p. e57405. doi: 10.1371/journal.pone.0057405 https://archive-ouverte.unige.ch/unige:72772

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Deposited on : 2015-05-26

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