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Adjuvant effects of CpG oligodeoxynucleotides on responses against T-independent type 2 antigens

Bozzotti, P
Davis, HL
Krieg, AM
Published in Immunology. 2001, vol. 102, no. 1, p. 67-76
Abstract Oligodeoxynucleotides containing CpG motifs (CpG-ODN) are potent in vitro B-cell activators and they have been successfully used to increase in vivo antibody responses to T-dependent peptide and protein antigens. In contrast, the use of CpG-ODN to enhance in vivo antibody responses to various T-independent type 2 (TI-2) antigens has recently generated contradictory results. In this study, we compared the CpG-ODN stimulatory effect on antibody responses of adult and young BALB/c mice to trinitrophenylaminoethyl-carboxymethyl (TNP) -Ficoll and to polysaccharides (PS) from several distinct serotypes of Streptococcus pneumoniae (SPn). CpG-ODN co-administration significantly enhanced antigen-specific immunoglobulin M (IgM), IgG, IgG1 and IgG2a titres to TNP-Ficoll. The depletion of CD4+ cells by monoclonal antibodies (GK1.5) identified their essential role in CpG-ODN-mediated enhancement of antibody responses. In contrast to TNP-Ficoll, CpG-ODN failed to enhance IgM and IgG responses to any of the 18 SPnPS serotypes tested. Providing T-cell epitopes by the conjugation of SPnPS to the carrier protein tetanus toxoid again allowed CpG-ODN to mediate enhancement of IgG, IgG2a and IgG3 responses to most SPnPS serotypes. Thus, antigen-presenting cell/T-cell interaction appears to largely mediate the in vivo influence of CpG-ODN on antibody responses to TI-2 antigens. In early life, additional factors limit CpG-ODN modulation of antibody responses to TI-2 antigens.
Keywords Adjuvants, ImmunologicAging/immunologyAnimalsAntibodies, Bacterial/biosynthesisAntigens, T-Independent/immunologyB-Lymphocytes/immunologyCD4-Positive T-Lymphocytes/immunologyCarrier Proteins/immunologyCpG Islands/immunologyFicoll/analogs & derivatives/immunologyHaptens/immunologyImmunoglobulin G/biosynthesisImmunoglobulin M/biosynthesisLymphocyte Activation/immunologyMiceMice, Inbred BALB COligonucleotides/immunologyPneumococcal Vaccines/immunologyStreptococcus pneumoniae/immunologyTrinitrobenzenes/immunology
PMID: 11168639
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Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
(ISO format)
KOVARIK, Jiri et al. Adjuvant effects of CpG oligodeoxynucleotides on responses against T-independent type 2 antigens. In: Immunology, 2001, vol. 102, n° 1, p. 67-76. doi: 10.1046/j.1365-2567.2001.01158.x https://archive-ouverte.unige.ch/unige:72696

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Deposited on : 2015-05-19

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