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Title

Induction of adult-like antibody, Th1, and CTL responses to measles hemagglutinin by early life murine immunization with an attenuated vaccinia-derived NYVAC(K1L) viral vector

Authors
Bozzotti, P
Wild, T
Published in Virology. 2001, vol. 285, no. 1, p. 12-20
Abstract Although initially developed in adult animals, novel viral vectors expressing recombinant measles antigens must eventually prove their success in the early life setting, where the efficacy of the currently used live-attenuated measles virus vaccine is limited. The immunological requirements for vaccine candidates include the generation of protective antibody responses as well as the induction of Th1 and cytotoxic T lymphocytes (CTL) responses, which is challenging in the neonatal setting. Here, we report that young BALB/c mice immunized with a single dose of a vaccinia-based NYVAC(K1L) vector generate adult-like antihemagglutinin (HA) antibody responses as well as adult-like Th1 and CTL responses. Despite this strong immunogenicity in early life, antibody responses (but not T-cell responses) to a single dose of NYVAC(K1L)-HA remained susceptible to inhibition by preexisting measles antibodies, calling for use of prime-boost strategies. NYVAC(K1L)-HA is the first attenuated live viral vector demonstrated as capable of inducing adult-like antibody, Th1, and CTL responses against measles in an early life murine immunization model, a capacity previously only reported for measles DNA vaccines.
Keywords AnimalsAnimals, NewbornAntibodies, Viral/analysisDisease Models, AnimalFemaleGenetic VectorsHemagglutinins, Viral/immunologyHumansImmunization, SecondaryMaleMeasles/immunology/prevention & controlMeasles Vaccine/administration & dosageMiceMice, Inbred BALB CMorbillivirus/immunologySpecific Pathogen-Free OrganismsT-Lymphocytes, Cytotoxic/immunologyTh1 Cells/immunologyTime FactorsVaccines, Attenuated/administration & dosageVaccinia virus/genetics
Identifiers
PMID: 11414801
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Article (Published version) (168 Kb) - public document Free access
Structures
Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
Citation
(ISO format)
KOVARIK, Jiri et al. Induction of adult-like antibody, Th1, and CTL responses to measles hemagglutinin by early life murine immunization with an attenuated vaccinia-derived NYVAC(K1L) viral vector. In: Virology, 2001, vol. 285, n° 1, p. 12-20. https://archive-ouverte.unige.ch/unige:72695

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Deposited on : 2015-05-19

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