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Title

Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray

Authors
de Souza Luna, L. K.
Heiser, Volker
Panning, Marcus
Drexler, J. F.
Mulangu, Sabue
Poon, Leo
Baumgarte, Sigrid
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Published in Journal of Clinical Microbiology. 2007, vol. 45, no. 3, p. 1049-1052
Abstract A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
Keywords Bacterial Typing TechniquesCoronavirus/ classification/genetics/isolation & purificationCoronavirus 229E, Human/classification/genetics/isolation & purificationCoronavirus OC43, Human/classification/genetics/isolation & purificationHumansOligonucleotide Array Sequence Analysis/ methodsRNA, Viral/analysis/geneticsReverse Transcriptase Polymerase Chain Reaction/ methodsSARS Virus/classification/genetics/isolation & purificationSpecies Specificity
Identifiers
PMID: 17229859
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DE SOUZA LUNA, L. K. et al. Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray. In: Journal of Clinical Microbiology, 2007, vol. 45, n° 3, p. 1049-1052. https://archive-ouverte.unige.ch/unige:7156

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Deposited on : 2010-06-21

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