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Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system

Join-Lambert, Olivier
Faurisson, Francois
Curty, L. K.
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Published in Journal of Bacteriology. 2002, vol. 184, no. 11, p. 3027-3033
Abstract Pseudomonas aeruginosa is an important opportunistic pathogen that produces a variety of cell-associated and secreted virulence factors. P. aeruginosa infections are difficult to treat effectively because of the rapid emergence of antibiotic-resistant strains. In this study, we analyzed whether the amoeba Dictyostelium discoideum can be used as a simple model system to analyze the virulence of P. aeruginosa strains. The virulent wild-type strain PAO1 was shown to inhibit growth of D. discoideum. Isogenic mutants deficient in the las quorum-sensing system were almost as inhibitory as the wild type, while rhl quorum-sensing mutants permitted growth of Dictyostelium cells. Therefore, in this model system, factors controlled by the rhl quorum-sensing system were found to play a central role. Among these, rhamnolipids secreted by the wild-type strain PAO1 could induce fast lysis of D. discoideum cells. By using this simple model system, we predicted that certain antibiotic-resistant mutants of P. aeruginosa should show reduced virulence. This result was confirmed in a rat model of acute pneumonia. Thus, D. discoideum could be used as a simple nonmammalian host system to assess pathogenicity of P. aeruginosa.
Keywords AnimalsBacterial Proteins/geneticsBacteriolysis/drug effectsDNA-Binding Proteins/deficiency/geneticsDictyostelium/growth & development/ microbiologyDisease Models, AnimalGlycolipids/pharmacologyHexosyltransferases/deficiency/geneticsMaleMethodsMutationPseudomonas Infections/microbiologyPseudomonas aeruginosa/genetics/ pathogenicityRatsRats, Sprague-DawleyTrans-Activators/deficiency/genetics
PMID: 12003944
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Research group Phagocytose (140)
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COSSON, Pierre et al. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. In: Journal of bacteriology, 2002, vol. 184, n° 11, p. 3027-3033. https://archive-ouverte.unige.ch/unige:7155

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Deposited on : 2010-06-21

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