Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system

Cosson, Pierre; Zulianello, Laurence; Join-Lambert, Olivier; Faurisson, Francois; Gebbie, Leigh; Benghezal, Mohammed; Van Delden, Christian; Curty, L. K.; Kohler, Thilo

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Title		Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system
Authors		Cosson, Pierre Zulianello, Laurence Join-Lambert, Olivier Faurisson, Francois Gebbie, Leigh Benghezal, Mohammed Van Delden, Christian Curty, L. K. Kohler, Thilo show all authors [1 - 9]
Published in		Journal of Bacteriology. 2002, vol. 184, no. 11, p. 3027-3033
Abstract		Pseudomonas aeruginosa is an important opportunistic pathogen that produces a variety of cell-associated and secreted virulence factors. P. aeruginosa infections are difficult to treat effectively because of the rapid emergence of antibiotic-resistant strains. In this study, we analyzed whether the amoeba Dictyostelium discoideum can be used as a simple model system to analyze the virulence of P. aeruginosa strains. The virulent wild-type strain PAO1 was shown to inhibit growth of D. discoideum. Isogenic mutants deficient in the las quorum-sensing system were almost as inhibitory as the wild type, while rhl quorum-sensing mutants permitted growth of Dictyostelium cells. Therefore, in this model system, factors controlled by the rhl quorum-sensing system were found to play a central role. Among these, rhamnolipids secreted by the wild-type strain PAO1 could induce fast lysis of D. discoideum cells. By using this simple model system, we predicted that certain antibiotic-resistant mutants of P. aeruginosa should show reduced virulence. This result was confirmed in a rat model of acute pneumonia. Thus, D. discoideum could be used as a simple nonmammalian host system to assess pathogenicity of P. aeruginosa.
Keywords		Animals — Bacterial Proteins/genetics — Bacteriolysis/drug effects — DNA-Binding Proteins/deficiency/genetics — Dictyostelium/growth & development/ microbiology — Disease Models, Animal — Glycolipids/pharmacology — Hexosyltransferases/deficiency/genetics — Male — Methods — Mutation — Pseudomonas Infections/microbiology — Pseudomonas aeruginosa/genetics/ pathogenicity — Rats — Rats, Sprague-Dawley — Trans-Activators/deficiency/genetics
Identifiers		PMID: 12003944
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC135065/pdf/1435.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research group		Phagocytose (140)
Citation (ISO format)		COSSON, Pierre et al. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. In: Journal of Bacteriology, 2002, vol. 184, n° 11, p. 3027-3033. https://archive-ouverte.unige.ch/unige:7155