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Scientific Article
unige:7085 
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Does protein kinase C control receptor-mediated phagocytosis in human neutrophils? |
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| Authors | ||
| Published in | FEBS Letters. 1988, vol. 239, no. 2, p. 371-375 | |
| Abstract | It was previously demonstrated that C3bi- but not IgG-mediated phagocytosis in human neutrophils is a Ca2+-independent process [(1985) Nature 315, 509-511]. The objective of this study was to elucidate the nature of an additional messenger signal(s) that may be involved in receptor-mediated phagocytosis in these cells. The C3bi- and IgG-mediated phagocytic ability of neutrophils was inhibited by forskolin, a potent activator of adenylate cyclase. It was found that forskolin induced a 3-fold increase in cAMP levels and simultaneously reduced the uptake of both C3bi- and IgG-opsonized particles by 65%. This inhibition was reversed by exposure to either phorbol myristate acetate (PMA) or diacylglycerol (OAG), which are both activators of protein kinase C, but not by the inactive analog 4 alpha-PMA. PMA could also restore the phagocytic ability of neutrophils with an experimentally impaired calcium response. These results suggest that activation of protein kinase C might be an important transducing signal controlling receptor-mediated phagocytosis in human neutrophils. | |
| Keywords | Cyclic AMP/blood — Diglycerides/pharmacology — Humans — Neutrophils/drug effects/immunology/ physiology — Phagocytosis — Protein Kinase C/ blood/physiology — Receptors, Immunologic/ physiology — Tetradecanoylphorbol Acetate/pharmacology | |
| Identifiers | PMID: 2846363 | |
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| Citation (ISO format) | ANDERSSON, T. et al. Does protein kinase C control receptor-mediated phagocytosis in human neutrophils?. In: FEBS Letters, 1988, vol. 239, n° 2, p. 371-375. https://archive-ouverte.unige.ch/unige:7085 |
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