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Title

Evaluation of the influence of protein precipitation prior to on-line SPE-LC-API/MS procedures using multivariate data analysis

Authors
Marchi, Ivano
Selman, Maurice
Published in Journal of Chromatography. B. 2007, vol. 845, no. 2, p. 244-52
Abstract Matrix effects on mass spectrometry (MS) response were investigated with three atmospheric pressure ionization (API) sources after on-line solid-phase extraction (SPE) of human plasma. On-line SPE was evaluated with one restricted access material (RAM), two large particle supports (LPS) and one monolith. A sample protein precipitation (PP) with acetonitrile (2:1) and a direct injection were tested. Principal component analysis (PCA) was performed to simplify data presentation and interpretation. Protein precipitation was found to be mandatory for reducing signal modification. Regarding sensitivity towards matrix effects after PP, atmospheric pressure photoionization (APPI) was globally the least sensitive ionization mode while electrospray ionization ESI was the most sensitive.
Keywords Alanine/analogs & derivativesBlood Proteins/isolation & purificationChemical PrecipitationCholecalciferolChromatography, Liquid/methodsFlunitrazepamFluoxetine/analogs & derivativesMass Spectrometry/methodsMethadoneMultivariate AnalysisOnline SystemsPyrrolidinesSolid Phase Extraction/methods
Identifiers
PMID: 17030156
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MARCHI, Ivano et al. Evaluation of the influence of protein precipitation prior to on-line SPE-LC-API/MS procedures using multivariate data analysis. In: Journal of Chromatography. B, 2007, vol. 845, n° 2, p. 244-52. https://archive-ouverte.unige.ch/unige:6876

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Deposited on : 2010-06-15

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