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Chromatin techniques for plant cells

Bowler, Chris
Benvenuto, Giovanna
Laflamme, Pierre
Molino, Diana
Probst, Aline V.
Tariq, Muhammad
Published in Plant Journal. 2004, vol. 39, no. 5, p. 776-89
Abstract A large number of recent studies have demonstrated that many important aspects of plant development are regulated by heritable changes in gene expression that do not involve changes in DNA sequence. Rather, these regulatory mechanisms involve modifications of chromatin structure that affect the accessibility of target genes to regulatory factors that can control their expression. The central component of chromatin is the nucleosome, containing the highly conserved histone proteins that are known to be subject to a wide range of post-translational modifications, which act as recognition codes for the binding of chromatin-associated factors. In addition to these histone modifications, DNA methylation can also have a dramatic influence on gene expression. To accommodate the burgeoning interest of the plant science community in the epigenetic control of plant development, a series of methods used routinely in our laboratories have been compiled that can facilitate the characterization of putative chromatin-binding factors at the biochemical, molecular and cellular levels.
Keywords Chromatin/metabolismDNA MethylationImmunoprecipitationMethodsPlants/cytology/genetics/metabolism
PMID: 15315638
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BOWLER, Chris et al. Chromatin techniques for plant cells. In: Plant Journal, 2004, vol. 39, n° 5, p. 776-89. doi: 10.1111/j.1365-313X.2004.02169.x https://archive-ouverte.unige.ch/unige:6861

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Deposited on : 2010-06-15

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