Glucose-6-phosphate dehydrogenase activity in spinach as measured by image analysis: A new approach for plant enzyme histochemistry

Crèvecoeur, Michèle; Cisse, Mbaye Mbengue; Albe, Xavier; Greppin, Hubert

doi:10.1007/BF02331424

Scientific article

English

Glucose-6-phosphate dehydrogenase activity in spinach as measured by image analysis: A new approach for plant enzyme histochemistry

Contributors

Crèvecoeur, Michèle; Cisse, Mbaye Mbengue; Albe, Xavier; Greppin, Hubert

Published in

Histochemical journal, vol. 28, no. 1, p. 25-32

Publication date

1996

Abstract

A relatively low-cost computer-assisted image analysis system is described. Software has been specifically written for the continuous monitoring of absorbance readings on cryostat sectons of plant tissues incubated in media to reveal enzyme activities. The equipment was tested by quantify ing glucose-6-phosphate dehydrogenase activity in cryostat sections from shoot apices of spinach plants. The reaction rate of the dehydrogenase activity was monitored at two incubation temperatures, 20°C and 30°C. Control incubations were performed in media lacking substrate. The specific test minus control reaction at 30°C was twice that at 20°C. Variation of the substrate concentration at 30°C yielded a Km value of 0.37 mM. These preliminary results show that our image analysis system can be used for kineti measurements of dehydrogenase activity in froizen tssue sections and constitute a new approach for enzyme histochemistry in the shoot apicla meristem.

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département des sciences végétales

Citation (ISO format)

CRÈVECOEUR, Michèle et al. Glucose-6-phosphate dehydrogenase activity in spinach as measured by image analysis: A new approach for plant enzyme histochemistry. In: Histochemical journal, 1996, vol. 28, n° 1, p. 25–32. doi: 10.1007/BF02331424

Article (Published version)

Identifiers

PID : unige:6860
DOI : 10.1007/BF02331424

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02331424

Journal ISSN

0018-2214

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Glucose-6-phosphate dehydrogenase activity in spinach as measured by image analysis: A new approach for plant enzyme histochemistry

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

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Additional URL for this publication

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Technical informations

Creation

First validation

Update

Status update

Last indexation