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Scientific article
English

Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin

Contributors

Tariq, Muhammad; Saze, Hidetoshi; Probst, Aline V.; Lichota, Jacek; Habu, Yoshiki; Paszkowski, Jerzy

Published in

Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 15, p. 8823-8827

Publication date

2003

Abstract

In mammals and plants, formation of heterochromatin is associated with hypermethylation of DNA at CpG sites and histone H3 methylation at lysine 9. Previous studies have revealed that maintenance of DNA methylation in Neurospora and Arabidopsis requires histone H3 methylation. A feedback loop from DNA methylation to histone methylation, however, is less understood. Its recent examination in Arabidopsis with a partial loss of function in DNA methyltransferase 1 (responsible for maintenance of CpG methylation) yielded conflicting results. Here we report that complete removal of CpG methylation in an Arabidopsis mutant null for DNA maintenance methyltransferase results in a clear loss of histone H3 methylation at lysine 9 in heterochromatin and also at heterochromatic loci that remain transcriptionally silent. Surprisingly, these dramatic alterations are not reflected in heterochromatin relaxation.

Keywords

  • Arabidopsis/genetics/metabolism
  • Arabidopsis Proteins
  • CpG Islands
  • DNA (Cytosine-5-)-Methyltransferase/genetics/metabolism
  • DNA Methylation
  • DNA, Plant/chemistry/genetics
  • Heterochromatin/chemistry/genetics
  • Histones/chemistry/genetics
  • Lysine/chemistry

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

TARIQ, Muhammad et al. Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin. In: Proceedings of the National Academy of Sciences of the United States of America, 2003, vol. 100, n° 15, p. 8823–8827. doi: 10.1073/pnas.1432939100

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0027-8424
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Technical informations

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08/06/2010 15:06:00

First validation

08/06/2010 15:06:00

Update

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