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Scientific article
English

Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin

Publication date2003
Abstract

In mammals and plants, formation of heterochromatin is associated with hypermethylation of DNA at CpG sites and histone H3 methylation at lysine 9. Previous studies have revealed that maintenance of DNA methylation in Neurospora and Arabidopsis requires histone H3 methylation. A feedback loop from DNA methylation to histone methylation, however, is less understood. Its recent examination in Arabidopsis with a partial loss of function in DNA methyltransferase 1 (responsible for maintenance of CpG methylation) yielded conflicting results. Here we report that complete removal of CpG methylation in an Arabidopsis mutant null for DNA maintenance methyltransferase results in a clear loss of histone H3 methylation at lysine 9 in heterochromatin and also at heterochromatic loci that remain transcriptionally silent. Surprisingly, these dramatic alterations are not reflected in heterochromatin relaxation.

Keywords
  • Arabidopsis/genetics/metabolism
  • Arabidopsis Proteins
  • CpG Islands
  • DNA (Cytosine-5-)-Methyltransferase/genetics/metabolism
  • DNA Methylation
  • DNA, Plant/chemistry/genetics
  • Heterochromatin/chemistry/genetics
  • Histones/chemistry/genetics
  • Lysine/chemistry
Affiliation Not a UNIGE publication
Citation (ISO format)
TARIQ, Muhammad et al. Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin. In: Proceedings of the National Academy of Sciences of the United States of America, 2003, vol. 100, n° 15, p. 8823–8827. doi: 10.1073/pnas.1432939100
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0027-8424
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