Scientific article

Mitogen-activated protein kinase phosphatase is required for genotoxic stress relief in Arabidopsis

Published inGenes & development, vol. 15, no. 6, p. 699-709
Publication date2001

Genotoxic stress activates complex cellular responses allowing for the repair of DNA damage and proper cell recovery. Although plants are exposed constantly to increasing solar UV irradiation, the signaling cascades activated by genotoxic environments are largely unknown. We have identified an Arabidopsis mutant (mkp1) hypersensitive to genotoxic stress treatments (UV-C and methyl methanesulphonate) due to disruption of a gene that encodes an Arabidopsis homolog of mitogen-activated protein kinase phosphatase (AtMKP1). Growth of the mkp1 mutant under standard conditions is indistinguishable from wild type, indicating a stress-specific function of AtMKP1. MAP kinase phosphatases (MKPs), the potent inactivators of MAP kinases, are considered important regulators of MAP kinase signaling. Although biochemical data from mammalian cell cultures suggests an involvement of MKPs in cellular stress responses, there is no in vivo genetic support for this view in any multicellular organism. The genetic and biochemical data presented here imply a central role for a MAP kinase cascade in genotoxic stress signaling in plants and indicate AtMKP1 to be a crucial regulator of the MAP kinase activity in vivo, determining the outcome of the cellular reaction and the level of genotoxic resistance.

  • Amino Acid Sequence
  • Arabidopsis/enzymology/genetics
  • Arabidopsis Proteins
  • DNA, Complementary/metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Immunoblotting
  • Lycopersicon esculentum/genetics
  • MAP Kinase Signaling System
  • Methyl Methanesulfonate
  • Mitogen-Activated Protein Kinases/genetics/physiology
  • Models, Genetic
  • Molecular Sequence Data
  • Mutagenesis
  • Mutagens
  • Mutation
  • Phenotype
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Ultraviolet Rays
  • Zea mays/genetics
Affiliation Not a UNIGE publication
Citation (ISO format)
ULM, Roman et al. Mitogen-activated protein kinase phosphatase is required for genotoxic stress relief in Arabidopsis. In: Genes & development, 2001, vol. 15, n° 6, p. 699–709. doi: 10.1101/gad.192601
Main files (1)
Article (Published version)
ISSN of the journal0890-9369

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