UNIGE document Scientific Article
previous document  unige:6798  next document
add to browser collection
Title

Long term in vitro-cultured plant cells show typical neoplastic features at the cytological level

Authors
Wüest, Jean
Published in Biology of the Cell. 2003, vol. 95, no. 6, p. 357-64
Abstract Cells from a green normal (dependent on exogenous hormones) callus and from an achlorophyllous fully habituated (independent from exogenous hormones) callus, both generated from the same sugarbeet strain more than twenty years ago, were reexamined cytologically, ten years after the first comparative description. Cells from the habituated callus, already considered as neoplastic cells, because terminating a neoplastic progression where the organogenic totipotency was lost, still showed nuclear invaginations, polynucleolation, vacuolation of nucleoli and incomplete cell walls, nevertheless at a higher degree. The present study particularly shows that, compared to their previous description, normal cells have started to acquire some features (polynucleolation, nuclear invaginations.) that are typical of the neoplastic cells. This suggests that normal cells, on the long term, also entered a neoplastic progression, which should explain the known progressive loss of regeneration capacity of too long subcultured hormone-dependent calli.
Keywords Beta vulgaris/cytology/ultrastructureCell LineCell Nucleus/metabolismCell Wall/metabolismMicroscopy, ElectronMicroscopy, Electron, ScanningPlant Growth Regulators/metabolismPlant Leaves/cytology/ultrastructurePlant TumorsPlants/cytology/ultrastructureStarch/metabolismTime Factors
Identifiers
PMID: 14519552
Full text
Article (Published version) (634 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
HASLER, Julien et al. Long term in vitro-cultured plant cells show typical neoplastic features at the cytological level. In: Biology of the Cell, 2003, vol. 95, n° 6, p. 357-64. https://archive-ouverte.unige.ch/unige:6798

115 hits

0 download

Update

Deposited on : 2010-06-08

Export document
Format :
Citation style :