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Title

Identification of the pharmacophore of the CC chemokine-binding proteins Evasin-1 and -4 using phage display

Authors
Dunn, Steven M
Rousseau, François
Dyer, Douglas
Shaw, Jeffrey
Power, Christine
Handel, Tracy
Proudfoot, Amanda
Published in Journal of Biological Chemistry. 2014, vol. 289, no. 46, p. 31846-31855
Abstract To elucidate the ligand-binding surface of the CC chemokine-binding proteins Evasin-1 and Evasin-4, produced by the tick Rhipicephalus sanguineus, we sought to identify the key determinants responsible for their different chemokine selectivities by expressing Evasin mutants using phage display. We first designed alanine mutants based on the Evasin-1·CCL3 complex structure and an in silico model of Evasin-4 bound to CCL3. The mutants were displayed on M13 phage particles, and binding to chemokine was assessed by ELISA. Selected variants were then produced as purified proteins and characterized by surface plasmon resonance analysis and inhibition of chemotaxis. The method was validated by confirming the importance of Phe-14 and Trp-89 to the inhibitory properties of Evasin-1 and led to the identification of a third crucial residue, Asn-88. Two amino acids, Glu-16 and Tyr-19, were identified as key residues for binding and inhibition of Evasin-4. In a parallel approach, we identified one clone (Y28Q/N60D) that showed a clear reduction in binding to CCL3, CCL5, and CCL8. It therefore appears that Evasin-1 and -4 use different pharmacophores to bind CC chemokines, with the principal binding occurring through the C terminus of Evasin-1, but through the N-terminal region of Evasin-4. However, both proteins appear to target chemokine N termini, presumably because these domains are key to receptor signaling. The results also suggest that phage display may offer a useful approach for rapid investigation of the pharmacophores of small inhibitory binding proteins.
Keywords Alanine/chemistryAmino Acid SequenceAnimalsCell MovementChemokine CCL3/chemistryChemokine CCL5/chemistry/geneticsChemokine CCL8/chemistryChemokines, CC/chemistryChemotaxisCrystallography, X-RayEnzyme-Linked Immunosorbent AssayGlycosylationHEK293 CellsHumansLigandsMolecular Sequence DataMutagenesis, Site-DirectedPeptide LibraryProtein BindingProtein Structure, TertiaryReceptors, Chemokine/chemistryRhipicephalus sanguineusSequence Homology, Amino AcidSurface Plasmon Resonance
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PMID: 25266725
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Structures
Project FP7: TIMER
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BONVIN, Pauline et al. Identification of the pharmacophore of the CC chemokine-binding proteins Evasin-1 and -4 using phage display. In: Journal of Biological Chemistry, 2014, vol. 289, n° 46, p. 31846-31855. https://archive-ouverte.unige.ch/unige:55994

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Deposited on : 2015-05-04

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