en
Scientific article
English

Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines

Published inEuropean Journal of Immunology, vol. 32, no. 3, p. 752-760
Publication date2002
Abstract

The capacity to induce high-avidity antibodies following early-life immunization has long been questioned, and the possibility of inducing such antibodies soon after birth is a recognized goal for a number of vaccination strategies. Therefore, we assessed the capacity to develop high-avidity antibodies to peptidic vaccines in 1-week-old BALB/c mice. The dynamics of the generation of antibody molecules of increasing avidity were analyzed on circulating serum antibodies and, where feasible, at the single-cell level on spleen and bone marrow antibody-secreting cells. Two alum-adsorbed protein-based human vaccines, tetanus toxoid (TT) and pertussis toxin, induced neonatal antibody responses with adult-like avidity profiles. This was confirmed at the level of spleen and bone marrow ASC. In contrast, immunization with TT-P30, a 21-mer synthetic peptide containing a TT-immunodominant epitope, trinitrophenyl hapten (TNP) conjugated to ovalbumin or TNP conjugated to Ficoll, induced a much lower avidity profile in early life than in adults. These observations indicate that in murine models the avidity maturation of T cell-dependent antibody responses induced by structurally complex protein vaccines can be fully elicited after early life immunization, as opposed to the maturation of responses induced with short peptides or hapten-based vaccines.

Keywords
  • Aging/immunology
  • Animals
  • Animals, Suckling/immunology
  • Antibodies, Bacterial/biosynthesis/immunology
  • Antibody Affinity
  • Bordetella pertussis/immunology
  • Clostridium tetani/immunology
  • Ficoll/analogs & derivatives/immunology
  • Immune System/growth & development
  • Immunization
  • Immunization Schedule
  • Immunodominant Epitopes/immunology
  • Immunoglobulin G/biosynthesis/immunology
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin/immunology
  • Peptide Fragments/immunology
  • Specific Pathogen-Free Organisms
  • Tetanus Toxoid/administration & dosage/immunology
  • Toxoids/administration & dosage/immunology
  • Trinitrobenzenes/immunology
  • Vaccines, Subunit/administration & dosage/immunology
Citation (ISO format)
SCHALLERT, Nadine et al. Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines. In: European Journal of Immunology, 2002, vol. 32, n° 3, p. 752–760. doi: 10.1002/1521-4141(200203)32:3<752::AID-IMMU752>3.0.CO;2-5
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Article (Published version)
accessLevelRestricted
ISSN of the journal0014-2980
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