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Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines

Schallert, Nadine
Bozzotti, Paola
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Published in European Journal of Immunology. 2002, vol. 32, no. 3, p. 752-60
Abstract The capacity to induce high-avidity antibodies following early-life immunization has long been questioned, and the possibility of inducing such antibodies soon after birth is a recognized goal for a number of vaccination strategies. Therefore, we assessed the capacity to develop high-avidity antibodies to peptidic vaccines in 1-week-old BALB/c mice. The dynamics of the generation of antibody molecules of increasing avidity were analyzed on circulating serum antibodies and, where feasible, at the single-cell level on spleen and bone marrow antibody-secreting cells. Two alum-adsorbed protein-based human vaccines, tetanus toxoid (TT) and pertussis toxin, induced neonatal antibody responses with adult-like avidity profiles. This was confirmed at the level of spleen and bone marrow ASC. In contrast, immunization with TT-P30, a 21-mer synthetic peptide containing a TT-immunodominant epitope, trinitrophenyl hapten (TNP) conjugated to ovalbumin or TNP conjugated to Ficoll, induced a much lower avidity profile in early life than in adults. These observations indicate that in murine models the avidity maturation of T cell-dependent antibody responses induced by structurally complex protein vaccines can be fully elicited after early life immunization, as opposed to the maturation of responses induced with short peptides or hapten-based vaccines.
Keywords Aging/immunologyAnimalsAnimals, Suckling/immunologyAntibodies, Bacterial/biosynthesis/immunologyAntibody AffinityBordetella pertussis/immunologyClostridium tetani/immunologyFicoll/analogs & derivatives/immunologyImmune System/growth & developmentImmunizationImmunization ScheduleImmunodominant Epitopes/immunologyImmunoglobulin G/biosynthesis/immunologyMiceMice, Inbred BALB COvalbumin/immunologyPeptide Fragments/immunologySpecific Pathogen-Free OrganismsTetanus Toxoid/administration & dosage/immunologyToxoids/administration & dosage/immunologyTrinitrobenzenes/immunologyVaccines, Subunit/administration & dosage/immunology
PMID: 11870619
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Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
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SCHALLERT, Nadine et al. Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines. In: European Journal of Immunology, 2002, vol. 32, n° 3, p. 752-60. doi: 10.1002/1521-4141(200203)32:3<752::AID-IMMU752>3.0.CO;2-5 https://archive-ouverte.unige.ch/unige:55907

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Deposited on : 2015-04-29

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