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Title

Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions

Authors
Hartmer, Ralf
Jabs, Wolfgang
Stoermer, Carsten
Samii, Kaveh
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Published in Analytical and Bioanalytical Chemistry. 2015, vol. 407, no. 10, p. 2837-45
Abstract Hemoglobin disorder diagnosis is a complex procedure combining several analytical steps. Due to the lack of specificity of the currently used protein analysis methods, the identification of uncommon hemoglobin variants (proteoforms) can become a hard task to accomplish. The aim of this work was to develop a mass spectrometry-based approach to quickly identify mutated protein sequences within globin chain variants. To reach this goal, a top-down electron transfer dissociation mass spectrometry method was developed for hemoglobin β chain analysis. A diagnostic product ion list was established with a color code strategy allowing to quickly and specifically localize a mutation in the hemoglobin β chain sequence. The method was applied to the analysis of rare hemoglobin β chain variants and an (A)γ-β fusion protein. The results showed that the developed data analysis process allows fast and reliable interpretation of top-down electron transfer dissociation mass spectra by nonexpert users in the clinical area.
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PMID: 25753013
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Article (Published version) (1.8 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Chimie et protéomique clinique (270)
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COELHO GRACA, Didia et al. Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions. In: Analytical and Bioanalytical Chemistry, 2015, vol. 407, n° 10, p. 2837-45. https://archive-ouverte.unige.ch/unige:55517

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Deposited on : 2015-04-14

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