Glucocorticoids aggravate hyperoxia-induced lung injury through decreased nuclear factor-kappa B activity

Barazzone, Constance; Pagano Aurrand-Lions, Alessandra; Juge, Cristiana Enrica; Ruchonnet, Isabelle; Rochat, Anne-Françoise; Vesin, Christian; Donati, Yves Richard

doi:10.1152/ajplung.00239.2002

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Title		Glucocorticoids aggravate hyperoxia-induced lung injury through decreased nuclear factor-kappa B activity
Authors		Barazzone, Constance Pagano Aurrand-Lions, Alessandra Juge, Cristiana Enrica Ruchonnet, Isabelle Rochat, Anne-Françoise Vesin, Christian Donati, Yves Richard
Published in		American Journal of Physiology. Lung Cellular and Molecular Physiology. 2003, vol. 284, no. 1, p. L197-204
Abstract		We previously reported that exposure of mice to hyperoxia is characterized by extensive lung cell necrosis and apoptosis, mild inflammatory response, and elevated circulating levels of corticosterone. Administration of hydroxycortisone acetate during hyperoxia aggravated lung injury. Using adrenalectomized (ADX) and sham-operated (sham) mice, we studied the role of the glucocorticoids in hyperoxia-induced lung injury. Lung damage was attenuated in ADX mice as measured by lung weight and protein and cell content in bronchoalveolar lavage and as seen by light microscopy. Mortality was delayed by 10 h. Nuclear factor-kappaB (NF-kappaB) activity was significantly decreased in lungs of sham mice exposed to hyperoxia but was preserved in ADX mice. There was a correlation between NF-kappaB activity in ADX mice and decreased levels of IkappaBalpha. In contrast, activator protein-1 activity increased similarly in both groups of mice. Levels of interleukin-6 (IL-6), a transcriptional target of NF-kappaB, were higher in bronchoalveolar lavage and serum of ADX than sham mice. However, the protective effect of ADX was not mediated by IL-6, because administration of recombinant human IL-6 to sham mice did not prevent lung damage. These results demonstrate that the adrenal response aggravates alveolar injury and is likely to be mediated by the decrease of NF-kappaB function involved in cell survival.
Keywords		Adrenalectomy — Animals — Apoptosis — Corticosterone/metabolism — Glucocorticoids/pharmacology — Hyperoxia/metabolism/pathology — I-kappa B Proteins/metabolism — Interleukin-6/pharmacology — Lung/drug effects/metabolism/pathology — Mice — Mice, Inbred C57BL — NF-kappa B/antagonists & inhibitors — Proline/analogs & derivatives/pharmacology — Pulmonary Alveoli — Pulmonary Edema/prevention & control — Thiocarbamates/pharmacology — Thymus Gland/pathology/physiopathology — Transcription Factor AP-1/metabolism
Identifiers		DOI: 10.1152/ajplung.00239.2002 PMID: 12388343
Full text		Article (Published version) (290 Kb) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research group		Facteurs influençants le développement pulmonaire: étude translationnelle chez l'animal et l'homme (182)
Citation (ISO format)		BARAZZONE, Constance et al. Glucocorticoids aggravate hyperoxia-induced lung injury through decreased nuclear factor-kappa B activity. In: American Journal of Physiology. Lung Cellular and Molecular Physiology, 2003, vol. 284, n° 1, p. L197-204. doi: 10.1152/ajplung.00239.2002 https://archive-ouverte.unige.ch/unige:55336