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Article scientifique
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Inhaled NO inhibits platelet aggregation and elevates plasma but not intraplatelet cGMP in healthy human volunteers

Publié dansAmerican journal of physiology. Heart and circulatory physiology, vol. 285, no. 2, p. H637-642
Date de publication2003
Résumé

Effects of inhaled nitric oxide (NO) on human platelet function are controversial. It is uncertain whether intraplatelet cGMP mediates the effect of inhaled NO on platelet function. We investigated the effect of 30 ppm inhaled NO on platelet aggregation and plasma and intraplatelet cGMP in 12 subjects. We performed platelet aggregation studies by using a photooptical aggregometer and five agonists (ADP, collagen, epinephrine, arachidonic acid, and ristocetin). During inhalation, the maximal extent of platelet aggregation decreased by 75% with epinephrine (P < 0.005), 56% with collagen (P < 0.005), and 20% with arachidonic acid (P < 0.05). Responses to ADP (8% P > 0.05) and ristocetin (5% P > 0.05) were unaffected. Platelet aggregation velocity decreased by 64% with collagen (P < 0.005), 60% with epinephrine (P < 0.05), 33% with arachidonic acid (P < 0.05), and 14% with ADP (P > 0.05). Plasma cGMP levels increased from 2.58 +/- 0.43 to 9.99 +/- 5.57 pmol/ml (P < 0.005), intraplatelet cGMP levels were unchanged (means +/- SD: 1.96 +/- 0.58 vs. 2.71 +/- 1.67 pmol/109 platelets; P > 0.05). Inhaled NO inhibits platelet aggregation via a cGMP independent mechanism.

Mots-clés
  • Administration, Inhalation
  • Adult
  • Blood Platelets/drug effects/metabolism
  • Cyclic GMP/blood
  • Female
  • Hemoglobins
  • Humans
  • Male
  • Middle Aged
  • Nitric Oxide/administration & dosage
  • Platelet Aggregation/drug effects
  • Platelet Count
Citation (format ISO)
BEGHETTI, Maurice et al. Inhaled NO inhibits platelet aggregation and elevates plasma but not intraplatelet cGMP in healthy human volunteers. In: American journal of physiology. Heart and circulatory physiology, 2003, vol. 285, n° 2, p. H637–642. doi: 10.1152/ajpheart.00622.2002
Fichiers principaux (1)
Article (Published version)
accessLevelPublic
Identifiants
ISSN du journal0363-6135
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260téléchargements

Informations techniques

Création03.03.2015 13:13:00
Première validation03.03.2015 13:13:00
Heure de mise à jour14.03.2023 23:09:24
Changement de statut14.03.2023 23:09:24
Dernière indexation16.01.2024 17:40:49
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