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Title

Unresponsiveness to lymphoid-mediated signals at the neonatal follicular dendritic cell precursor level contributes to delayed germinal center induction and limitations of neonatal antibody responses to T-dependent antigens

Authors
Bozzotti, Paola
Duchosal, Michel A
Published in Journal of Immunology. 2003, vol. 170, no. 6, p. 2824-32
Abstract The factors limiting neonatal and infant IgG Ab responses to T-dependent Ags are only partly known. In this study, we assess how these B cell responses are influenced by the postnatal development of the spleen and lymph node microarchitecture. When BALB/c mice were immunized with alum-adsorbed tetanus toxoid at various stages of their immune development, a major functional maturation step for induction of serum IgG, Ab-secreting cells, and germinal center (GC) responses was identified between the second and the third week of life. This correlated with the development of the follicular dendritic cell (FDC) network, as mature FDC clusters only appeared at 2 wk of age. Adoptive transfer of neonatal splenocytes into adult SCID mice rapidly induced B cell follicles and FDC precursor differentiation into mature FDC, indicating effective recruitment and signaling capacity of neonatal B cells. In contrast, adoptive transfer of adult splenocytes into neonatal SCID mice induced primary B cell follicles without any differentiation of mature FDC and failed to correct limitations of tetanus toxoid-induced GC. Thus, unresponsiveness to lymphoid-mediated signals at the level of neonatal FDC precursors delays FDC maturation and GC induction, thus limiting primary Ab-secreting cell responses to T-dependent Ags in early postnatal life.
Keywords Aging/immunologyAnimalsAnimals, Newborn/growth & development/immunologyAntibodies, Bacterial/biosynthesisAntibody-Producing Cells/cytology/secretionB-Lymphocyte Subsets/cytology/immunologyCell Differentiation/immunologyCell Movement/immunologyChemokine CXCL13Chemokines, CXC/physiologyDendritic Cells, Follicular/cytology/immunologyFemaleGerminal Center/cytology/immunologyHaptens/immunologyImmune Tolerance/immunologyLymphocyte Subsets/cytology/immunologyMiceMice, Inbred BALB CMice, SCIDOvalbumin/immunologyReceptors, CXCR5Receptors, ChemokineReceptors, Cytokine/physiologySignal Transduction/immunologyStem Cells/cytology/immunologyTetanus Toxoid/immunologyTrinitrobenzenes/immunology
Identifiers
PMID: 12626532
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Article (Published version) (649 Kb) - public document Free access
Structures
Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
Citation
(ISO format)
PIHLGREN BOSCH, Maria Astrid Louise et al. Unresponsiveness to lymphoid-mediated signals at the neonatal follicular dendritic cell precursor level contributes to delayed germinal center induction and limitations of neonatal antibody responses to T-dependent antigens. In: Journal of Immunology, 2003, vol. 170, n° 6, p. 2824-32. https://archive-ouverte.unige.ch/unige:55268

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Deposited on : 2015-04-08

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