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Scientific article
English

Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat

Contributors

Nguyen, Tuan Huy; Birraux, Jacques Maurice; Wildhaber, Barbara; Myara, Anne; Trivin, Francois; Le Coultre, Claude Pierrette; Trono, Didier; Chardot, Christophe

Published in

Transplantation, vol. 82, no. 6, p. 794-803

Publication date

2006

Abstract

Ex vivo liver gene therapy provides an attractive alternative to orthotopic liver transplantation for the treatment of liver diseases. We previously reported a protocol in which human primary hepatocytes are highly transduced in Suspension with Lentiviral vectors and Immediately Transplanted (SLIT). Here, we evaluated the SLIT approach in Gunn rats, the animal model for Crigler-Najjar syndrome type 1, a defect in bilirubin UDP-glucuronosyltransferase (BUGT).

Keywords

  • Animals
  • Base Sequence
  • Cell Line
  • Disease Models, Animal
  • Genetic Vectors
  • Green Fluorescent Proteins/genetics
  • HeLa Cells
  • Hepatocytes/transplantation/virology
  • Humans
  • Hyperbilirubinemia/surgery
  • Kidney
  • Lentivirus/genetics
  • Liver/virology
  • Male
  • Promoter Regions, Genetic
  • RNA, Messenger/genetics/isolation & purification
  • RNA, Viral/genetics/isolation & purification
  • Rats
  • Rats, Gunn
  • Transplantation, Isogeneic

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

NGUYEN, Tuan Huy et al. Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat. In: Transplantation, 2006, vol. 82, n° 6, p. 794–803. doi: 10.1097/01.tp.0000234675.56598.35

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0041-1337
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1downloads

Technical informations

Creation

29/01/2015 15:08:00

First validation

29/01/2015 15:08:00

Update

14/03/2023 23:05:18

Status update

14/03/2023 23:05:18

Last indexation

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