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Title

Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat

Authors
Myara, Anne
Trivin, Francois
Published in Transplantation. 2006, vol. 82, no. 6, p. 794-803
Abstract Ex vivo liver gene therapy provides an attractive alternative to orthotopic liver transplantation for the treatment of liver diseases. We previously reported a protocol in which human primary hepatocytes are highly transduced in Suspension with Lentiviral vectors and Immediately Transplanted (SLIT). Here, we evaluated the SLIT approach in Gunn rats, the animal model for Crigler-Najjar syndrome type 1, a defect in bilirubin UDP-glucuronosyltransferase (BUGT).
Keywords AnimalsBase SequenceCell LineDisease Models, AnimalGenetic VectorsGreen Fluorescent Proteins/geneticsHeLa CellsHepatocytes/transplantation/virologyHumansHyperbilirubinemia/surgeryKidneyLentivirus/geneticsLiver/virologyMalePromoter Regions, GeneticRNA, Messenger/genetics/isolation & purificationRNA, Viral/genetics/isolation & purificationRatsRats, GunnTransplantation, Isogeneic
Identifiers
PMID: 17006327
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Structures
Research group Recherche clinique en chirurgie pédiatrique (886)
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NGUYEN, Tuan Huy et al. Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat. In: Transplantation, 2006, vol. 82, n° 6, p. 794-803. https://archive-ouverte.unige.ch/unige:54944

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Deposited on : 2015-03-31

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