UNIGE document Scientific Article - Review
previous document  unige:5427  next document
add to browser collection
Title

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes

Authors
Galluzzi, L.
Aaronson, S A.
Abrams, J.
Alnemri, E S.
Andrews, D W.
Baehrecke, E H.
Bazan, N G.
Blagosklonny, M V.
show hidden authors show all authors [1 - 79]
Published in Cell Death and Differentiation. 2009, vol. 16, no. 8, p. 1093-107
Abstract Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
Keywords ApoptosisCell DeathEukaryotic Cells/cytologyFlow CytometryGuidelines as TopicHumansImmunoblottingMicroscopy, Electron, ScanningMicroscopy, FluorescenceSpectrometry, Fluorescence
Identifiers
PMID: 19373242
Full text
Structures
Research group Les mitochondries dans la vie cellulaire (850)
Citation
(ISO format)
GALLUZZI, L. et al. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. In: Cell Death and Differentiation, 2009, vol. 16, n° 8, p. 1093-107. https://archive-ouverte.unige.ch/unige:5427

209 hits

5 downloads

Update

Deposited on : 2010-03-16

Export document
Format :
Citation style :