UNIGE document Scientific Article
previous document  unige:5418  next document
add to browser collection
Title

The double-stranded RNA binding domain of the vaccinia virus E3L protein inhibits both RNA- and DNA-induced activation of interferon beta

Authors
Published in Journal of Biological Chemistry. 2009, vol. 284, no. 38, p. 25471-8
Abstract Vaccinia virus, a large DNA virus that replicates in the cytoplasm, expresses its E3L protein to inhibit the cellular innate immune response and apoptosis. E3L is a bifunctional protein that contains an N-terminal DNA binding domain (BD) and a C-terminal double-stranded RNA (dsRNA)-BD (residues 100-190), both of which contribute to viral pathogenesis by blocking the activation of cellular genes that respond to the viral infection. We report that expression of the dsRNA-BD alone inhibits not only the dsRNA-induced activation of interferon beta (IFNbeta) but also that of 5'-triphosphate single-stranded RNA and DNA-induced IFNbeta activation even though E3L(100-190) does not bind the latter two pathogen-associated molecular patterns. This inhibition occurs in both human HeLa and A549 cells, where RIG-I appears to be required for dsDNA-induced IFNbeta activation. Unexpectedly, the two residues most important for dsRNA binding are also critical for this domain's ability to inhibit all three nucleic acid-induced cellular responses.
Keywords AnimalsDEAD-box RNA Helicases/genetics/metabolismDNA, Viral/genetics/metabolismHela CellsHumansInterferon-beta/genetics/metabolismMiceProtein Binding/geneticsProtein Structure, TertiaryRNA, Double-StrandedRNA, Viral/genetics/metabolismRNA-Binding Proteins/genetics/metabolismVaccinia/genetics/metabolismVaccinia virus/genetics/metabolism/pathogenicityViral Proteins/genetics/metabolism
Identifiers
PMID: 19584049
Full text
Article (Published version) (2.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jbc.org/content/284/38/25471.long
Structures
Research group Virologie moléculaire (275)
Citation
(ISO format)
MARQ, Jean-Baptiste et al. The double-stranded RNA binding domain of the vaccinia virus E3L protein inhibits both RNA- and DNA-induced activation of interferon beta. In: Journal of Biological Chemistry, 2009, vol. 284, n° 38, p. 25471-8. https://archive-ouverte.unige.ch/unige:5418

195 hits

0 download

Update

Deposited on : 2010-03-16

Export document
Format :
Citation style :