Early assessment of apoptosis in isolated islets of Langerhans

Cattan, P; Berney, Thierry; Schena, S; Molano, R D; Pileggi, A; Vizzardelli, C; Ricordi, C; Inverardi, L

doi:10.1097/00007890-200104150-00006

Scientific article

English

Early assessment of apoptosis in isolated islets of Langerhans

ContributorsCattan, P; Berney, Thierry; Schena, S; Molano, R D; Pileggi, A; Vizzardelli, C; Ricordi, C; Inverardi, L

Published inTransplantation, vol. 71, no. 7, p. 857-862

Publication date2001

Abstract

There is substantial evidence to link early graft loss after islet transplantation to isolation-induced islet cell apoptosis. Measurement of caspase 3 activity and detection of the lost cell membrane asymmetry, revealed by annexin V binding, are newly available assays that allow the analysis of early events of apoptosis.

Keywords

Animals
Annexin A5/metabolism
Apoptosis
Biological Markers
Caspase 3
Caspases/metabolism
DNA Fragmentation
Electrophoresis, Polyacrylamide Gel
In Situ Nick-End Labeling
In Vitro Techniques
Islets of Langerhans/cytology/metabolism/physiology
Rats
Rats, Inbred Lew

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de chirurgie

Research groups

La transplantation d'îlots de Langerhans (623)

Citation (ISO format)

CATTAN, P et al. Early assessment of apoptosis in isolated islets of Langerhans. In: Transplantation, 2001, vol. 71, n° 7, p. 857–862. doi: 10.1097/00007890-200104150-00006

Article (Accepted version)

Identifiers

PID : unige:47546
DOI : 10.1097/00007890-200104150-00006
PMID : 11349716

Journal ISSN0041-1337

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Early assessment of apoptosis in isolated islets of Langerhans

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