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Title

Hexavalent capsomers of herpes simplex virus type 2 : symmetry, shape, dimensions, and oligomeric status

Authors
Steven, Alasdair C.
Roberts, C. R.
Hay, J
Bisher, ME
Trus, Benes L.
Published in Journal of Virology. 1986, vol. 57, no. 2, p. 578-584
Abstract The structures of the hexavalent capsomers of herpes simplex virus type 2 were analyzed by negative staining electron microscopy of capsomer patches derived from partially disrupted nucleocapsids. Optimally computer-averaged images were formed for each of the three classes of capsomer distinguished by their respective positions on the surface of the icosahedral capsid with a triangulation number of 16; in projection, each capsomer exhibited unequivocal sixfold symmetry. According to correspondence analysis of our set of capsomer images, no significant structural differences were detected among the three classes of capsomers, as visualized under these conditions. Taking into account information from images of freeze-dried, platinum-shadowed nucleocapsid fragments, it was established that each hexavalent capsomer is a hexamer of the 155-kilodalton major capsid protein. The capsomer has the form of a sixfold hollow cone approximately 12 nm in diameter and approximately 15 nm in depth, whose axial channel tapers in width from the outside towards the inner capsid surface.
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Structures
Research groups Computer Vision and Multimedia Laboratory
Multimodal Interaction Group
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STEVEN, Alasdair C. et al. Hexavalent capsomers of herpes simplex virus type 2 : symmetry, shape, dimensions, and oligomeric status. In: Journal of Virology, 1986, vol. 57, n° 2, p. 578-584. https://archive-ouverte.unige.ch/unige:47450

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