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Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans

Molano, R D
Pileggi, A
Cattan, P
Vizzardelli, C
Ricordi, C
Inverardi, L
Published in Cell transplantation. 2001, vol. 10, no. 7, p. 633-7
Abstract A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p = 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-alpha release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-alpha: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.
Keywords AnimalsDiabetes Mellitus, Type 1/therapyFemaleGraft Survival/immunologyIslets of Langerhans Transplantation/immunologyMacrophage Colony-Stimulating Factor/geneticsMacrophages/immunologyMaleMiceMice, Inbred C57BLMice, Mutant StrainsOsteopetrosis/genetics/immunology
PMID: 11714198
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Research group La transplantation d'îlots de Langerhans (623)
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BERNEY, Thierry et al. Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. In: Cell transplantation, 2001, vol. 10, n° 7, p. 633-7. doi: 10.3727/000000001783986341 https://archive-ouverte.unige.ch/unige:47277

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Deposited on : 2015-02-25

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