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Scientific article
English

Status of p53 in first-trimester cytotrophoblastic cells

Contributors

Cohen, Marie-Benoîte; Meisser, A; Haenggeli, L; Irminger-Finger, I; Bischof, Paul

Published in

Molecular human reproduction, vol. 13, no. 2, p. 111-116

Publication date

2007

Abstract

p53 has been called the cellular gatekeeper of the genome because it can induce cell-cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. As p53 has been observed in first-trimester cytotrophoblastic cells (CTB), but its expression in normal cells is generally not detectable because of its short half-life, p53 could play an important role in cellular differentiation and/or in the control of the invasion of trophoblastic cells; therefore, p53 status was investigated in these cells. Using different antibodies recognizing different epitopes of p53 protein, abundant p53 expression was observed both in nuclear and in cytoplasmic compartments of first-trimester CTB. Whereas p53 was detected in the nuclei of few trophoblastic cells with an antibody recognizing the N-terminal epitope of the protein, high expression level of p53 in the cytoplasm of CTB was detected with an antibody recognizing the middle part of p53. The lack of immunoreactivity of p53 with antibodies recognizing the epitopes located at the N-terminus of p53 and the high level of p53 protein observed in the cytoplasm of CTB suggest that the N-terminus of p53 is involved in the formation of complexes. These cytoplasmic complexes were detected under non-reducing conditions in western blot analysis and had apparent molecular weights (MW) of 195, 167 or 125 kDa. These complexes could prolong the half-life of p53 in the cytoplasm of CTBs. By contrast, in the nuclei of CTBs, p53 seems to be present as a tetramer.

Keywords

  • Cells, Cultured
  • Dimerization
  • Female
  • Humans
  • Immunohistochemistry
  • Pregnancy
  • Pregnancy Trimester, First/metabolism
  • Protein Isoforms
  • Protein Structure, Tertiary
  • Trophoblasts/cytology/metabolism
  • Tumor Suppressor Protein p53/chemistry/metabolism

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

COHEN, Marie-Benoîte et al. Status of p53 in first-trimester cytotrophoblastic cells. In: Molecular human reproduction, 2007, vol. 13, n° 2, p. 111–116. doi: 10.1093/molehr/gal105

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

1360-9947
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Technical informations

Creation

17/02/2015 16:27:00

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17/02/2015 16:27:00

Update

14/03/2023 22:50:55

Status update

14/03/2023 22:50:54

Last indexation

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