UNIGE document Scientific Article
previous document  unige:46641  next document
add to browser collection
Title

The immunomodulating properties of human respiratory syncytial virus and immunostimulating complexes containing Quillaja saponin components QH-A, QH-C and ISCOPREP703

Authors
Hu, Ke-Fei
Chen, Margaret
Lövgren Bengtsson, K
Morein, Bror
Published in FEMS Immunology and Medical Microbiology. 2005, vol. 43, no. 2, p. 269-76
Abstract A successful vaccine against human RSV (HRSV) is likely to induce a Th1 or a balanced Th1/TH2 cytokine response. We tested a panel of HRSV immunostimulating complexes (ISCOMs) containing different Quillaja saponin fractions (QH-A, QH-C, and 703: a mixture of 70% QH-A and 30% QH-C) with different immunological properties for their capacity of inducing innate and acquired immune responses. The HRSV 703 ISCOMs induced the strongest innate and acquired immune responses, followed by RSV QH-C and QH-A ISCOMs. All three formulations induced various degrees of Th1 bias response with prominent production of IFN-gamma being 10-50 times higher than that of IL-4 and IL-5. The HRSV specific IgG isotype profile correlated with the predominant secretion of Th1 cytokines, with strong induction of IgG2a antibodies. The 703 ISCOMs induced the most pronounced Th1 profile followed by QH-C and QH-A ISCOMs. The high incorporation of F protein in these ISCOMs compared to G protein combined with the Th1 biased nature of ISCOM are likely to be the causes to promote a Th1 type of profile. The prospect to formulate an RSV ISCOM formulation with an optimal Th1/Th2 balance is in reach particularly in view of the versatile properties of the ISCOM concept.
Keywords Adjuvants, ImmunologicAnimalsAntibodies, Viral/bloodFemaleISCOMs/immunologyImmunoglobulin G/bloodInterferon-gamma/analysisInterleukin-4/analysisInterleukin-5/analysisMiceQuillaja/chemistry/immunologyRespiratory Syncytial Virus Infections/prevention & controlRespiratory Syncytial Virus Vaccines/immunologyRespiratory Syncytial Virus, Human/immunologyVaccination/methodsViral Proteins/immunology
Identifiers
PMID: 15681158
Full text
Article (Published version) (373 Kb) - public document Free access
Structures
Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
Citation
(ISO format)
HU, Ke-Fei et al. The immunomodulating properties of human respiratory syncytial virus and immunostimulating complexes containing Quillaja saponin components QH-A, QH-C and ISCOPREP703. In: FEMS Immunology and Medical Microbiology, 2005, vol. 43, n° 2, p. 269-76. https://archive-ouverte.unige.ch/unige:46641

177 hits

91 downloads

Update

Deposited on : 2015-02-12

Export document
Format :
Citation style :