en
Scientific article
English

Modulation of the in vivo primate anti-Gal response through administration of anti-idiotypic antibodies

Published inXenotransplantation, vol. 9, no. 2, p. 106-114
Publication date2002
Abstract

Polyclonal anti-idiotypic antibodies (AIA) were generated against human Gal alpha 1,3Gal antibodies (anti-Gal) isolated from a single donor. Specificity of the AIA was demonstrated by selective binding to anti-Gal antibodies (Ab) and absence of reactivity to non-Gal Ab. The idiotopes identified by AIA were present on anti-Gal Ab from all of the human samples evaluated (n=59) as well as on pooled samples, demonstrating that a restricted number of dominant idiotopes characterized the human anti-Gal Ab response. Furthermore, the AIA had cross-species reactivity with baboon serum samples (n=19), suggesting that the overall shape of the anti-Gal Ab combining site is conserved throughout the Old World primates and providing additional evidence of the limited heterogeneity of the anti-Gal Ab repertoire. In order to evaluate the potential effect of AIA in the modulation of the anti-Gal response in vivo, a baboon was injected with repeated doses of the purified AIA. Following AIA treatment, new Ab were generated that reduced Ab-mediated cytotoxicity to porcine cells. Furthermore, administration of the AIA to a baboon prolonged the survival of intravenously infused pig hematopoietic cells when compared with their survival in a control baboon that did not receive prior AIA treatment but underwent a similar conditioning regimen.

Keywords
  • Animals
  • Antibodies, Anti-Idiotypic/administration & dosage/immunology
  • Disaccharides/immunology
  • Graft Rejection/immunology/prevention & control
  • Hematopoietic Stem Cell Transplantation
  • Humans
  • Papio
  • Swine
  • Swine, Miniature
  • Transplantation Immunology
  • Transplantation, Heterologous
Citation (ISO format)
MCMORROW, Isabel M et al. Modulation of the in vivo primate anti-Gal response through administration of anti-idiotypic antibodies. In: Xenotransplantation, 2002, vol. 9, n° 2, p. 106–114. doi: 10.1034/j.1399-3089.2002.1o028.x
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0908-665X
483views
0downloads

Technical informations

Creation02.02.2015 16:03:00
First validation02.02.2015 16:03:00
Update time14.03.2023 22:47:53
Status update14.03.2023 22:47:52
Last indexation16.01.2024 16:34:59
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack