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Modulation of the in vivo primate anti-Gal response through administration of anti-idiotypic antibodies

McMorrow, Isabel M
Treter, Sarah
Neethling, Francisca A
Alwayn, Ian P J
Comrack, Christopher A
Kitamura, Hiroshi
Awwad, Michel
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Published in Xenotransplantation. 2002, vol. 9, no. 2, p. 106-14
Abstract Polyclonal anti-idiotypic antibodies (AIA) were generated against human Gal alpha 1,3Gal antibodies (anti-Gal) isolated from a single donor. Specificity of the AIA was demonstrated by selective binding to anti-Gal antibodies (Ab) and absence of reactivity to non-Gal Ab. The idiotopes identified by AIA were present on anti-Gal Ab from all of the human samples evaluated (n=59) as well as on pooled samples, demonstrating that a restricted number of dominant idiotopes characterized the human anti-Gal Ab response. Furthermore, the AIA had cross-species reactivity with baboon serum samples (n=19), suggesting that the overall shape of the anti-Gal Ab combining site is conserved throughout the Old World primates and providing additional evidence of the limited heterogeneity of the anti-Gal Ab repertoire. In order to evaluate the potential effect of AIA in the modulation of the anti-Gal response in vivo, a baboon was injected with repeated doses of the purified AIA. Following AIA treatment, new Ab were generated that reduced Ab-mediated cytotoxicity to porcine cells. Furthermore, administration of the AIA to a baboon prolonged the survival of intravenously infused pig hematopoietic cells when compared with their survival in a control baboon that did not receive prior AIA treatment but underwent a similar conditioning regimen.
Keywords AnimalsAntibodies, Anti-Idiotypic/administration & dosage/immunologyDisaccharides/immunologyGraft Rejection/immunology/prevention & controlHematopoietic Stem Cell TransplantationHumansPapioSwineSwine, MiniatureTransplantation ImmunologyTransplantation, Heterologous
PMID: 11897003
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Research group Cultures cellulaires et transplantations (519)
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MCMORROW, Isabel M et al. Modulation of the in vivo primate anti-Gal response through administration of anti-idiotypic antibodies. In: Xenotransplantation, 2002, vol. 9, n° 2, p. 106-14. doi: 10.1034/j.1399-3089.2002.1o028.x https://archive-ouverte.unige.ch/unige:46264

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Deposited on : 2015-02-03

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