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Mouse neutrophils express the decoy type 2 interleukin-1 receptor (IL-1R2) constitutively and in acute inflammatory conditions

Martin, Praxedis
Talabot-Ayer, Dominique
Rose-John, Stefan
Chalaris, Athena
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Published in Journal of leukocyte biology. 2013, vol. 94, no. 4, p. 791-802
Abstract The proinflammatory activities of IL-1 are tightly controlled at different levels. IL-1R2 acts as a decoy receptor and has been shown to regulate the biological effects of IL-1 in vitro and in vivo. However, little is known about its natural expression in the mouse in physiologic and pathologic conditions. In this study, we examined IL-1R2 mRNA and protein expression in isolated cells and tissues in response to different stimulatory conditions. Data obtained using ex vivo CD11b(+)Ly6G(+) peripheral blood cells and in vitro-differentiated CD11b(+)Ly6G(+) BMG indicated that neutrophils are the major source of constitutively expressed IL-1R2 in the mouse. The expression of IL-1R2 on BMG and ex vivo Ly6G(+) peripheral blood cells was highly up-regulated by HC. IL-1R2 pull-down experiments showed that mouse rIL-1β binds to BMG IL-1R2, whereas binding of IL-1Ra could not be detected. Furthermore, LPS treatment induced shedding of IL-1R2 from the neutrophil membrane in vitro and in vivo, executed mainly by ADAM17. Finally, in in vivo models of inflammation, including thioglycolate-induced acute peritonitis and acute lung injury, infiltrating Ly6G(+) neutrophils, expressed IL-1R2. Our data show that in the mouse, neutrophils mainly express the decoy receptor IL-1R2 under naïve and inflammatory conditions. These data suggest that neutrophils may contribute to the resolution of acute inflammation.
Keywords Acute DiseaseAnimalsBone Marrow Cells/drug effects/metabolism/pathologyHydrocortisone/pharmacologyInflammation/metabolism/pathologyInterleukin 1 Receptor Antagonist Protein/metabolismInterleukin-1beta/metabolismLipopolysaccharides/pharmacologyMiceMice, Inbred C57BLNeutrophils/drug effects/metabolismProtein Binding/drug effectsReceptors, Interleukin-1 Type II/metabolismUp-Regulation/drug effects
PMID: 23817563
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Research group Mécanisme de l'inflammation articulaire (44)
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MARTIN, Praxedis et al. Mouse neutrophils express the decoy type 2 interleukin-1 receptor (IL-1R2) constitutively and in acute inflammatory conditions. In: Journal of leukocyte biology, 2013, vol. 94, n° 4, p. 791-802. doi: 10.1189/jlb.0113035 https://archive-ouverte.unige.ch/unige:46007

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Deposited on : 2015-01-29

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