UNIGE document Scientific Article
previous document  unige:45995  next document
add to browser collection
Title

Skin flap-induced regression of granulation tissue correlates with reduced growth factor and increased metalloproteinase expression

Authors
Darby, Ian A
Bisucci, Teresa
Published in The Journal of Pathology. 2002, vol. 197, no. 1, p. 117-27
Abstract Previous studies have shown that covering granulation tissue of a full-thickness skin wound by a vascularized skin flap induces tissue remodeling, with a rapid loss of granulation tissue cells by apoptosis. In the present study, in situ hybridization has been used to examine mRNA expression for several factors that may be implicated in the apoptosis seen in this tissue. Skin wounds were made on the dorsal skin of 8-week-old rats. Ten days after wounding, skin flaps were created surgically and sutured over the granulation tissue. Tissue sections of granulation tissue from various times after addition of the skin flap were hybridized with 33P-labelled cRNA probes for transforming growth factor-beta1 (TGF-beta1), beta-inducible gene H3 (beta-ig-h3), alpha1 (1) procollagen, alpha-smooth muscle actin, matrix metalloproteinase-13 (MMP-13) and -2 (MMP-2), tissue inhibitor of metalloproteinase-1 (TIMP-1), and inducible nitric oxide synthase (iNOS). Control granulation tissue prior to addition of the skin flap showed high levels of TGF-beta1, beta-ig-h3, alpha1 (1) procollagen, alpha-smooth muscle actin, and TIMP-1 expression. MMP-13, MMP-2, and iNOS mRNA were low in 10-day granulation tissue. Addition of a skin flap resulted in a decrease in the expression of TGF-beta1, beta-ig-h3, alpha1 (I) procollagen, alpha-smooth muscle actin, and TIMP-1, but increased expression of MMP-13 and MMP-2. Similarly, an increase in iNOS mRNA expression was observed in the granulation tissue after addition of the skin flap. Addition of a vascularized skin flap may result in rapid remodelling of granulation tissue due to a decrease in expression of the trophic growth factor TGF-beta1 and increased degradation of extracellular matrix due to an alteration in the balance between MMPs and their inhibitor, TIMP-1. Additionally, increased iNOS expression may also favour apoptosis through the generation of free radicals. The additive effect of reduced growth factor expression, increased extracellular matrix turnover, and nitric oxide generation may result in the fibroblast and vascular cell apoptosis seen during the rapid remodelling of this tissue.
Keywords AnimalsApoptosisFemaleGene ExpressionGranulation Tissue/metabolism/pathologyGrowth Substances/metabolismIn Situ HybridizationMetalloendopeptidases/metabolismNitric Oxide Synthase/metabolismRNA, Messenger/geneticsRatsRats, WistarSkin Transplantation/pathologySurgical Flaps/pathologyTissue Inhibitor of Metalloproteinase-1/genetics/metabolismTransforming Growth Factor beta/genetics/metabolismTransforming Growth Factor beta1
Identifiers
PMID: 12081196
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Pittet Cuenod Brigitte (chirurgie plastique et reconstructive) (96)
Citation
(ISO format)
DARBY, Ian A et al. Skin flap-induced regression of granulation tissue correlates with reduced growth factor and increased metalloproteinase expression. In: The Journal of Pathology, 2002, vol. 197, n° 1, p. 117-27. https://archive-ouverte.unige.ch/unige:45995

225 hits

0 download

Update

Deposited on : 2015-01-29

Export document
Format :
Citation style :