UNIGE document Scientific Article
previous document  unige:45075  next document
add to browser collection
Title

In vivo brain macromolecule signals in healthy and lioblastoma mouse models: 1H magnetic resonance spectroscopy, post-processing and metabolite quantification at 14.1 T

Authors
Craveiro, Mélanie
Clément-Schatlo, Virginie
Cudalbu, Cristina
Published in Journal of Neurochemistry. 2014, vol. 129, no. 5, p. 806-815
Abstract In 1H magnetic resonance spectroscopy, macromolecule signals underlay metabolite signals, the understanding of which is necessary for reliable metabolite quantification. When macromolecule signals are measured using an inversion-recovery pulse sequence, special care needs to be taken to correctly remove residual metabolite signals to obtain a pure macromolecule spectrum. Furthermore, since a single spectrum is commonly used for quantification in multiple experiments, the impact of potential macromolecule signal variability, due to regional differences or pathologies, on metabolite quantification has to be assessed. In this study, we introduced a novel method to post-process measured macromolecule signals that offers a flexible and robust way of removing residual metabolite signals. This method was applied to investigate regional differences in the mouse brain macromolecule signals that may affect metabolite quantification when not taken into account. However, since no significant differences in metabolite quantification were detected, it was concluded that a single macromolecule spectrum can be generally used for the quantification of healthy mouse brain spectra. Alternatively, the study of a mouse model of human glioma showed several alterations of the macromolecule spectrum, including, but not limited to, increased mobile lipids signals, which had to be taken into account to avoid significant metabolite quantification errors.
Identifiers
PMID: 24611713
Full text
Article (Published version) (808 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://doi.wiley.com/10.1111/jnc.12673
Structures
Citation
(ISO format)
CRAVEIRO, Mélanie et al. In vivo brain macromolecule signals in healthy and lioblastoma mouse models: 1H magnetic resonance spectroscopy, post-processing and metabolite quantification at 14.1 T. In: Journal of Neurochemistry, 2014, vol. 129, n° 5, p. 806-815. https://archive-ouverte.unige.ch/unige:45075

183 hits

0 download

Update

Deposited on : 2015-01-13

Export document
Format :
Citation style :