Anti-fibroblast antibodies detected by cell-based ELISA in systemic sclerosis enhance the collagenolytic activity and matrix metalloproteinase-1 production in dermal fibroblasts

Fineschi, S; Cozzi, F; Burger, Danielle; Dayer, J-M; Meroni, P L; Chizzolini, Carlo

doi:10.1093/rheumatology/kem241

Scientific article

English

Anti-fibroblast antibodies detected by cell-based ELISA in systemic sclerosis enhance the collagenolytic activity and matrix metalloproteinase-1 production in dermal fibroblasts

ContributorsFineschi, S; Cozzi, F; Burger, Danielle; Dayer, J-M; Meroni, P L; Chizzolini, Carlo

Published inRheumatology, vol. 46, no. 12, p. 1779-1785

Publication date2007

Abstract

Antibodies binding to the surface of fibroblasts (anti-fibroblast antibodies: AFA) have been described in systemic sclerosis (SSc). We aimed to assess the effect of AFA on extracellular matrix (ECM) turnover and whether AFA were associated with anti-topoisomerase-I antibody.

Keywords

Adult
Antibodies, Anti-Idiotypic/analysis/drug effects
Blotting, Western
Case-Control Studies
Cells, Cultured
Collagen/metabolism
Enzyme-Linked Immunosorbent Assay
Female
Fibroblasts/drug effects/immunology
Fluorescent Antibody Technique, Indirect
Humans
Immunoglobulin G/pharmacology
Interleukin-1/pharmacology
Male
Matrix Metalloproteinase 1/drug effects/metabolism
Middle Aged
RNA, Messenger/analysis
Scleroderma, Systemic/immunology/physiopathology
Skin/pathology
Tissue Inhibitor of Metalloproteinase-1/drug effects/metabolism

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Research groups

La matrice extracellulaire (651)

Citation (ISO format)

FINESCHI, S et al. Anti-fibroblast antibodies detected by cell-based ELISA in systemic sclerosis enhance the collagenolytic activity and matrix metalloproteinase-1 production in dermal fibroblasts. In: Rheumatology, 2007, vol. 46, n° 12, p. 1779–1785. doi: 10.1093/rheumatology/kem241

Article (Published version)

Identifiers

PID : unige:44555
DOI : 10.1093/rheumatology/kem241
PMID : 17982166

Journal ISSN1462-0324

489views

309downloads

Creation09/12/2014 10:43:00

First validation09/12/2014 10:43:00

Update14/03/2023 22:35:15

Status update14/03/2023 22:35:14

Last indexation30/10/2024 21:55:41

Archive ouverte UNIGE

Anti-fibroblast antibodies detected by cell-based ELISA in systemic sclerosis enhance the collagenolytic activity and matrix metalloproteinase-1 production in dermal fibroblasts

Antibodies binding to the surface of fibroblasts (anti-fibroblast antibodies: AFA) have been described in systemic sclerosis (SSc). We aimed to assess the effect of AFA on extracellular matrix (ECM) turnover and whether AFA were associated with anti-topoisomerase-I antibody.

Technical informations