UNIGE document Scientific Article
previous document  unige:44226  next document
add to browser collection

Cell cycle constraints on capsulation and bacteriophage susceptibility

Beaussart, Audrey
Theraulaz, Laurence
Dufrêne, Yves F
Published in eLife. 2014, vol. 3
Abstract Despite the crucial role of bacterial capsules in pathogenesis, it is still unknown if systemic cues such as the cell cycle can control capsule biogenesis. In this study, we show that the capsule of the synchronizable model bacterium Caulobacter crescentus is cell cycle regulated and we unearth a bacterial transglutaminase homolog, HvyA, as restriction factor that prevents capsulation in G1-phase cells. This capsule protects cells from infection by a generalized transducing Caulobacter phage (φCr30), and the loss of HvyA confers insensitivity towards φCr30. Control of capsulation during the cell cycle could serve as a simple means to prevent steric hindrance of flagellar motility or to ensure that phage-mediated genetic exchange happens before the onset of DNA replication. Moreover, the multi-layered regulatory circuitry directing HvyA expression to G1-phase is conserved during evolution, and HvyA orthologues from related Sinorhizobia can prevent capsulation in Caulobacter, indicating that alpha-proteobacteria have retained HvyA activity.
PMID: 25421297
Full text
Article (Published version) (5.1 MB) - public document Free access
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
(ISO format)
ARDISSONE, Silvia et al. Cell cycle constraints on capsulation and bacteriophage susceptibility. In: eLife, 2014, vol. 3. doi: 10.7554/eLife.03587 https://archive-ouverte.unige.ch/unige:44226

366 hits



Deposited on : 2014-12-23

Export document
Format :
Citation style :