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Title

Loss of heterozygosity for the short arm of chromosome 11 (11p15) in human milk epithelial cells immortalized by microinjection of SV40 DNA.

Authors
Brandt, D
Weintraub, J
Zhou, W G
Aapro, M
Published in Cancer Research. 1991, vol. 51, no. 1, p. 294-300
Abstract We have developed, by microinjection of SV40 DNA into human milk epithelial cells, a new mammary cell line, Hu-MI, which exhibits the phenotype of luminal cells or so-called "breast cancer precursor cells." This cell line retains the phenotype of primary cells as demonstrated by the expression of keratins 18 and 19 and of polymorphic epithelial mucins. However, the cells do not grow in agar after more than 80 passages, nor do they form tumors in nude mice. Established cells contain 2 copies of SV40 DNA integrated into the cellular genome and up to 14 copies of free SV40 DNA. A deletion of the short arm of chromosome 11 (11p15) including the c-Ha-ras and the beta-globin genes was found in the immortalized cells when the DNA from these cells was compared to the DNA from peripheral blood mononuclear cells obtained from the same donor. In addition, this cell line showed a good transfection efficiency for other DNA sequences using classical transfection and selection techniques with a neomycin resistance gene (pKOneo). Selective microinjection of DNA into tumor precursor cells may prove useful for the study of the molecular mechanisms involved in breast carcinogenesis. The possible significance of the loss of 11p13-15 in malignant progression of breast cancer is discussed.
Keywords AneuploidyBlotting, SouthernBreast/cytologyCell DivisionCell Membrane/immunologyCell Transformation, ViralChromosomes, Human, Pair 11DNA, Viral/geneticsEpithelial CellsGlobins/geneticsHeterozygoteHumansKeratins/metabolismPolymorphism, Restriction Fragment LengthProto-Oncogene Proteins/geneticsProto-Oncogene Proteins p21(ras)/geneticsReceptor, erbB-2Simian virus 40/genetics
Identifiers
PMID: 1703035
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GARCIA, Irène et al. Loss of heterozygosity for the short arm of chromosome 11 (11p15) in human milk epithelial cells immortalized by microinjection of SV40 DNA. In: Cancer Research, 1991, vol. 51, n° 1, p. 294-300. https://archive-ouverte.unige.ch/unige:44186

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