en
Scientific article
Open access
English

Establishment of two rabbit mammary epithelial cell lines with distinct oncogenic potential and differentiated phenotype after microinjection of transforming genes

Published inMolecular and cellular biology, vol. 6, no. 6, p. 1974-1982
Publication date1986
Abstract

The goal of this work was to establish an assay for transformation of epithelial cells. Two epithelial cell lines were obtained after microinjecting transforming genes into primary rabbit mammary secretory cells. The cell lines were analyzed for their oncogenic potential and for the maintenance of a differentiated phenotype. A fully transformed cell line, which retained epithelial cell organization, was obtained by coinjecting simian virus 40 DNA and the activated human c-Ha-ras gene. The proliferation rate of these cells was high, with a doubling time of 16 h. Their growth was anchorage independent, and they had lost contact inhibition. The cells were tumorigenic in nude mice, but had no metastatic potential. Both microinjected DNAs were efficiently transcribed and translated, in contrast to the casein genes, which were expressed in primary cells but not in the transformed cell line. An immortalized cell line established after injection with simian virus 40 DNA alone was characterized by a moderate rate of proliferation with a doubling time of approximately 30 h. The growth of these cells was contact inhibited and anchorage dependent. The cells were not tumorigenic in nude mice. The viral DNA was expressed during early passages, as shown by the presence of the large T antigen in cell nuclei, but not at later passages. A high number of lactogenic hormone receptors were found associated with the cell surface. Despite the presence of these receptors, no induction of genes coding for milk proteins was observed after addition of prolactin. These data demonstrate that this assay system can be used to assess the immortalizing and transforming potential of candidate oncogenes in epithelial cells.

Keywords
  • Animals
  • Caseins/genetics
  • Cell Differentiation
  • Cell Line
  • Cell Transformation, Viral
  • DNA, Neoplasm/genetics
  • DNA, Viral/genetics
  • Epithelial Cells
  • Gene Expression Regulation
  • Mammary Glands, Animal/cytology
  • Microscopy, Electron
  • Neoplasms, Experimental/genetics
  • Oncogenes
  • Rabbits
  • Simian virus 40
Citation (ISO format)
GARCIA, Irène et al. Establishment of two rabbit mammary epithelial cell lines with distinct oncogenic potential and differentiated phenotype after microinjection of transforming genes. In: Molecular and cellular biology, 1986, vol. 6, n° 6, p. 1974–1982. doi: 10.1128/mcb.6.6.1974
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0270-7306
516views
371downloads

Technical informations

Creation11/25/2014 4:40:00 PM
First validation11/25/2014 4:40:00 PM
Update time03/14/2023 10:32:37 PM
Status update03/14/2023 10:32:37 PM
Last indexation01/16/2024 3:28:29 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack